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10.1242/dev.146811 http://scihub22266oqcxt.onion/10.1242/dev.146811 C5560041!5560041!28765214     free     free     free       Development 2017 ; 144 (15): 2748-63 Nephropedia Template TP {{tp|p=28765214|t=2017. Epigenetic resetting of human pluripotency |pdf=|usr=}}{{28765214}} gab.com Text Epigenetic resetting of human pluripotency JO: Development http://www.kidney.de/pget.php?&tw=1&pmid=28765214 #FOAvip Much attention has focussed on the conversion of human pluripotent stem cells (PSCs) to a more naïve developmental status. Here we provide a method for resetting via transient histone deacetylase inhibition. The protocol is effective across multiple PSC lines and can proceed without karyotype change. Reset cells can be expanded without feeders with a doubling time of around 24?h. WNT inhibition stabilises the resetting process. The transcriptome of reset cells diverges markedly from that of primed PSCs and shares features with human inner cell mass (ICM). Reset cells activate expression of primate-specific transposable elements. DNA methylation is globally reduced to a level equivalent to that in the ICM and is non-random, with gain of methylation at specific loci. Methylation imprints are mostly lost, however. Reset cells can be re-primed to undergo tri-lineage differentiation and germline specification. In female reset cells, appearance of biallelic X-linked gene transcription indicates reactivation of the silenced X chromosome. On reconversion to primed status, XIST-induced silencing restores monoallelic gene expression. The facile and robust conversion routine with accompanying data resources will enable widespread utilisation, interrogation, and refinement of candidate naïve cells. Twit Text FOAVip Epigenetic resetting of human pluripotency ????Development http://www.kidney.de/pget.php?&tw=1&pmid=28765214 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28765214 #FOAvip English Wikipedia <ref>{{Cite pmid|28765214}}</ref> Epigenetic resetting of human pluripotency #MMPMID28765214 Guo G; von Meyenn F; Rostovskaya M; Clarke J; Dietmann S; Baker D; Sahakyan A; Myers S; Bertone P; Reik W; Plath K; Smith A Development 2017[Aug]; 144 (15): 2748-63 PMID28765214show ga Much attention has focussed on the conversion of human pluripotent stem cells (PSCs) to a more naïve developmental status. Here we provide a method for resetting via transient histone deacetylase inhibition. The protocol is effective across multiple PSC lines and can proceed without karyotype change. Reset cells can be expanded without feeders with a doubling time of around 24?h. WNT inhibition stabilises the resetting process. The transcriptome of reset cells diverges markedly from that of primed PSCs and shares features with human inner cell mass (ICM). Reset cells activate expression of primate-specific transposable elements. DNA methylation is globally reduced to a level equivalent to that in the ICM and is non-random, with gain of methylation at specific loci. Methylation imprints are mostly lost, however. Reset cells can be re-primed to undergo tri-lineage differentiation and germline specification. In female reset cells, appearance of biallelic X-linked gene transcription indicates reactivation of the silenced X chromosome. On reconversion to primed status, XIST-induced silencing restores monoallelic gene expression. The facile and robust conversion routine with accompanying data resources will enable widespread utilisation, interrogation, and refinement of candidate naïve cells. äEpigenetic resetting of human pluripotency (epmc).pdf DeepDyve Pubget Overpricing