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.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117 Int+J+Ophthalmol
2017 ; 10
(8
): 1195-1202
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MiR-200c suppresses the migration of retinoblastoma cells by reversing epithelial
mesenchymal transition
#MMPMID28861342
Shao XL
; Chen Y
; Gao L
Int J Ophthalmol
2017[]; 10
(8
): 1195-1202
PMID28861342
show ga
AIM: To analyze the relationship between clinical features and epithelial
mesenchymal transition (EMT) in retinoblastoma (RB), further to investigate
whether miR-200c regulates the EMT and migration of RB cells. METHODS: Expression
of EMT-related markers and tumor-related factors were detected by
immuno-histochemistry analysis in RB tissue from 29 cases. Correlations between
their expression and clinical characteristics were analyzed. The regulation
effects of miR-200c on EMT-related markers, tumor-related factors were observed
in mRNA level and protein level by real-time polymerase chain reaction (PCR) and
Western blot, respectively, in Y79 and Weri-rb1 cells. Its effects on migration
force of these RB cell lines were also detected with Transwell test. RESULTS:
Lower expression of E-cadherin was present in the cases with malignant prognosis.
MiR-200c promoted the expression of E-cadherin and decreased the expression of
Vimentin and N-cadherin in Y79 and Weri-rb1 cells. Migration force of RB cells
could be inhibited by miR-200c. CONCLUSION: EMT might be associated with bad
prognosis in RB. MiR-200c suppresses the migration of retinoblastomatous cells by
reverse EMT.