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10.1002/anie.201700570

http://scihub22266oqcxt.onion/10.1002/anie.201700570
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C5549273!5549273!28379629
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suck abstract from ncbi


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pmid28379629      Angew+Chem+Int+Ed+Engl 2017 ; 56 (22): 6131-5
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  • Characterization of plasma membrane ceramides by super-resolution microscopy #MMPMID28379629
  • Burgert A; Schlegel J; Bécam J; Doose PS; Bieberich E; Schubert-Unkmeir A; Sauer M
  • Angew Chem Int Ed Engl 2017[May]; 56 (22): 6131-5 PMID28379629show ga
  • The sphingolipid ceramide regulates cellular processes such as differentiation, proliferation, growth arrest and apoptosis. Ceramide-rich membrane areas promote structural changes within the plasma membrane which segregates membrane receptors and affect membrane curvature and vesicle formation, fusion and trafficking. Here, we label ceramides by immunocytochemistry and visualize their distribution on the plasma membrane of different cells with virtually molecular resolution by direct stochastic optical reconstruction microscopy (dSTORM). Super-resolution images show that independent of labeling conditions and cell type 50?60% of all membrane ceramides are located in ceramide-rich platforms (CRPs) with a size of ~ 75 nm which are composed of at least ~ 20 ceramides. Treatment of cells with Bacillus cereus sphingomyelinase (bSMase) increases the overall ceramide concentration in the plasma membrane, the quantity of CRPs and their size. Simultaneously, the ceramide concentration in CRPs increases approximately twofold.
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