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10.1038/nature21719 http://scihub22266oqcxt.onion/10.1038/nature21719 C5540373!5540373!28355179     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nature 2017 ; 544 (7648): 101-4 Nephropedia Template TP {{tp|p=28355179|t=2017. CRISPR-Cas systems exploit viral DNA injection to establish and maintain adaptive immunity |pdf=|usr=}}{{28355179}} gab.com Text CRISPR-Cas systems exploit viral DNA injection to establish and maintain adaptive immunity JO: Nature http://www.kidney.de/pget.php?&tw=1&pmid=28355179 #FOAvip CRISPR-Cas systems provide protection against viral1 and plasmid2 infection by capturing short DNA sequences from these invaders and integrating them into the CRISPR locus of the prokaryotic host1. These sequences, known as spacers, are transcribed into short RNA guides3?5 that specify the cleavage site of Cas nucleases in the genome of the invader6?8. When spacer sequences are acquired during viral infection is not known. To investigate this, we followed spacer acquisition in Staphylococcus aureus cells harboring a type II CRISPR-Cas9 system after infection with the staphylococcal bacteriophage ?12. We found that new spacers are acquired immediately following infection preferentially from the cos site, the viral free DNA end that is first injected into the cell. Analysis of spacer acquisition after infection with mutant phages demonstrated that most spacers are acquired during DNA injection, but not during other stages of the viral cycle that produce free DNA ends, such as DNA replication or packaging. Finally, we showed that spacers acquired from early-injected genomic regions, which direct Cas9 cleavage of the viral DNA immediately after infection, provide better immunity than spacers acquired from late-injected regions. Our results reveal that CRISPR-Cas systems exploit the phage life cycle to generate a pattern of spacer acquisition that ensures the success of the CRISPR immune response. Twit Text FOAVip CRISPR-Cas systems exploit viral DNA injection to establish and maintain adaptive immunity ????Nature http://www.kidney.de/pget.php?&tw=1&pmid=28355179 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28355179 #FOAvip English Wikipedia <ref>{{Cite pmid|28355179}}</ref> CRISPR-Cas systems exploit viral DNA injection to establish and maintain adaptive immunity #MMPMID28355179 Modell JW; Jiang W; Marraffini LA Nature 2017[Apr]; 544 (7648): 101-4 PMID28355179show ga CRISPR-Cas systems provide protection against viral1 and plasmid2 infection by capturing short DNA sequences from these invaders and integrating them into the CRISPR locus of the prokaryotic host1. These sequences, known as spacers, are transcribed into short RNA guides3?5 that specify the cleavage site of Cas nucleases in the genome of the invader6?8. When spacer sequences are acquired during viral infection is not known. To investigate this, we followed spacer acquisition in Staphylococcus aureus cells harboring a type II CRISPR-Cas9 system after infection with the staphylococcal bacteriophage ?12. We found that new spacers are acquired immediately following infection preferentially from the cos site, the viral free DNA end that is first injected into the cell. Analysis of spacer acquisition after infection with mutant phages demonstrated that most spacers are acquired during DNA injection, but not during other stages of the viral cycle that produce free DNA ends, such as DNA replication or packaging. Finally, we showed that spacers acquired from early-injected genomic regions, which direct Cas9 cleavage of the viral DNA immediately after infection, provide better immunity than spacers acquired from late-injected regions. Our results reveal that CRISPR-Cas systems exploit the phage life cycle to generate a pattern of spacer acquisition that ensures the success of the CRISPR immune response. äCRISPR-Cas systems exploit viral DNA injection to establish and mainta(epmc).pdf DeepDyve Pubget Overpricing