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10.1152/ajprenal.00054.2017 http://scihub22266oqcxt.onion/10.1152/ajprenal.00054.2017 C5538839!5538839 !28298358     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\28298358 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Am+J+Physiol+Renal+Physiol 2017 ; 313 (1 ): F30-F46 Nephropedia Template TP {{tp|p=28298358 |t=2017. Identification of ?-catenin-interacting proteins in nuclear fractions of native rat collecting duct cells |pdf=|usr=}}{{28298358 }} gab.com Text Identification of -catenin-interacting proteins in nuclear fractions of native rat collecting duct cells JO: Am J Physiol Renal Physiol http://www.kidney.de/pget.php?&tw=1&pmid=28298358 #FOAvip The gene encoding the aquaporin-2 water channel is regulated transcriptionally in response to vasopressin. In the renal collecting duct, vasopressin stimulates the nuclear translocation and phosphorylation (at Ser(552)) of ?-catenin, a multifunctional protein that acts as a transcriptional coregulator in the nucleus. The purpose of this study was to identify ?-catenin-interacting proteins that might be involved in transcriptional regulation in rat inner medullary collecting duct (IMCD) cells, using experimental and computational approaches. We used a standard chromatin immunoprecipitation procedure coupled to mass spectrometry (ChIP-MS) in a nuclear fraction isolated from rat IMCD suspensions. Over four biological replicates, we reproducibly identified 43 ?-catenin-binding proteins, including several known ?-catenin-binding partners as well as novel interacting proteins. Multiple proteins involved in transcriptional regulation were identified (Taf1, Jup, Tdrd3, Cdh1, Cenpj, and several histones). Many of the identified ?-catenin-binding partners were found in prior studies to translocate to the nucleus in response to vasopressin. There was only one DNA-binding transcription factor (TF), specifically Taf1, part of the RNA-polymerase II preinitiation complex. To identify sequence-specific TFs that might interact with ?-catenin, Bayes' theorem was used to integrate data from several information sources. The analysis identified several TFs with potential binding sites in the Aqp2 gene promoter that could interact with ?-catenin in the regulation of Aqp2 gene transcription, specifically Jun, Junb, Jund, Atf1, Atf2, Mef2d, Usf1, Max, Pou2f1, and Rxra. The findings provide information necessary for modeling the transcriptional response to vasopressin. Twit Text FOAVip Identification of -catenin-interacting proteins in nuclear fractions of native rat collecting duct cells ????Am J Physiol Renal Physiol http://www.kidney.de/pget.php?&tw=1&pmid=28298358 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28298358 #FOAvip English Wikipedia <ref>{{Cite pmid|28298358 }}</ref> Identification of ?-catenin-interacting proteins in nuclear fractions of native rat collecting duct cells #MMPMID28298358 Hwang JR ; Chou CL ; Medvar B ; Knepper MA ; Jung HJ Am J Physiol Renal Physiol 2017[Jul]; 313 (1 ): F30-F46 PMID28298358 show ga The gene encoding the aquaporin-2 water channel is regulated transcriptionally in response to vasopressin. In the renal collecting duct, vasopressin stimulates the nuclear translocation and phosphorylation (at Ser(552)) of ?-catenin, a multifunctional protein that acts as a transcriptional coregulator in the nucleus. The purpose of this study was to identify ?-catenin-interacting proteins that might be involved in transcriptional regulation in rat inner medullary collecting duct (IMCD) cells, using experimental and computational approaches. We used a standard chromatin immunoprecipitation procedure coupled to mass spectrometry (ChIP-MS) in a nuclear fraction isolated from rat IMCD suspensions. Over four biological replicates, we reproducibly identified 43 ?-catenin-binding proteins, including several known ?-catenin-binding partners as well as novel interacting proteins. Multiple proteins involved in transcriptional regulation were identified (Taf1, Jup, Tdrd3, Cdh1, Cenpj, and several histones). Many of the identified ?-catenin-binding partners were found in prior studies to translocate to the nucleus in response to vasopressin. There was only one DNA-binding transcription factor (TF), specifically Taf1, part of the RNA-polymerase II preinitiation complex. To identify sequence-specific TFs that might interact with ?-catenin, Bayes' theorem was used to integrate data from several information sources. The analysis identified several TFs with potential binding sites in the Aqp2 gene promoter that could interact with ?-catenin in the regulation of Aqp2 gene transcription, specifically Jun, Junb, Jund, Atf1, Atf2, Mef2d, Usf1, Max, Pou2f1, and Rxra. The findings provide information necessary for modeling the transcriptional response to vasopressin. |Animals [MESH] |Aquaporin 2/genetics/metabolism [MESH] |Bayes Theorem [MESH] |Binding Sites [MESH] |Cell Nucleus/drug effects/*metabolism [MESH] |Chromatin Immunoprecipitation [MESH] |Chromatography, Liquid [MESH] |Computational Biology [MESH] |Databases, Genetic [MESH] |Gene Expression Regulation [MESH] |Kidney Tubules, Collecting/cytology/drug effects/*metabolism [MESH] |Male [MESH] |Promoter Regions, Genetic [MESH] |Protein Binding [MESH] |Protein Interaction Maps [MESH] |Proteomics/methods [MESH] |Rats, Sprague-Dawley [MESH] |Systems Integration [MESH] |Tandem Mass Spectrometry [MESH] |Transcription Factors/genetics/metabolism [MESH] |Transcription, Genetic [MESH] |Vasopressins/pharmacology [MESH]Identification of ?-catenin-interacting proteins in nuclear fractions (epmc).pdf DeepDyve Pubget Overpricing