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10.1016/j.ymeth.2017.05.006 http://scihub22266oqcxt.onion/10.1016/j.ymeth.2017.05.006 C5531069!5531069 !28522325     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\28522325 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Methods 2017 ; 121-122 (ä): 138-145 Nephropedia Template TP {{tp|p=28522325 |t=2017. Generation of chromosomal translocations that lead to conditional fusion protein expression using CRISPR-Cas9 and homology-directed repair |pdf=|usr=}}{{28522325 }} gab.com Text Generation of chromosomal translocations that lead to conditional fusion protein expression using CRISPR-Cas9 and homology-directed repair JO: Methods http://www.kidney.de/pget.php?&tw=1&pmid=28522325 #FOAvip Recurrent chromosomal translocations often lead to expression of fusion proteins associated with oncogenic transformation. To study translocations and downstream events, genome editing techniques have been developed to generate chromosomal translocations through non-homologous end joining of DNA double-strand breaks introduced at the two participating endogenous loci. However, the frequencies at which these events occur is usually too low to efficiently clone cells carrying the translocation. This article provides a detailed method using CRISPR-Cas9 technology and homology-directed repair to efficiently isolate cells harboring a chromosomal translocation. For an additional level of control, the resulting fusion protein is conditionally expressed to allow early events in oncogenic transformation to be studied. We focus on the generation of the EWSR1-WT1 fusion using human mesenchymal cells, which is associated with the translocation found in desmoplastic small round cell tumors. Twit Text FOAVip Generation of chromosomal translocations that lead to conditional fusion protein expression using CRISPR-Cas9 and homology-directed repair ????Methods http://www.kidney.de/pget.php?&tw=1&pmid=28522325 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28522325 #FOAvip English Wikipedia <ref>{{Cite pmid|28522325 }}</ref> Generation of chromosomal translocations that lead to conditional fusion protein expression using CRISPR-Cas9 and homology-directed repair #MMPMID28522325 Vanoli F ; Jasin M Methods 2017[May]; 121-122 (ä): 138-145 PMID28522325 show ga Recurrent chromosomal translocations often lead to expression of fusion proteins associated with oncogenic transformation. To study translocations and downstream events, genome editing techniques have been developed to generate chromosomal translocations through non-homologous end joining of DNA double-strand breaks introduced at the two participating endogenous loci. However, the frequencies at which these events occur is usually too low to efficiently clone cells carrying the translocation. This article provides a detailed method using CRISPR-Cas9 technology and homology-directed repair to efficiently isolate cells harboring a chromosomal translocation. For an additional level of control, the resulting fusion protein is conditionally expressed to allow early events in oncogenic transformation to be studied. We focus on the generation of the EWSR1-WT1 fusion using human mesenchymal cells, which is associated with the translocation found in desmoplastic small round cell tumors. |*CRISPR-Cas Systems [MESH] |*Clustered Regularly Interspaced Short Palindromic Repeats [MESH] |Abdominal Neoplasms/*genetics/metabolism/pathology [MESH] |Bacterial Proteins/*genetics/metabolism [MESH] |CRISPR-Associated Protein 9 [MESH] |Cell Line [MESH] |Cell Transformation, Neoplastic/genetics/metabolism/pathology [MESH] |Chromosomes, Human, Pair 11 [MESH] |Chromosomes, Human, Pair 22 [MESH] |DNA Breaks, Double-Stranded [MESH] |Desmoplastic Small Round Cell Tumor/*genetics/metabolism/pathology [MESH] |Endonucleases/*genetics/metabolism [MESH] |Genome, Human [MESH] |Humans [MESH] |Mesenchymal Stem Cells/metabolism/pathology [MESH] |Mutagenesis, Site-Directed/methods [MESH] |Oncogene Proteins, Fusion/genetics/metabolism [MESH] |RNA, Guide, CRISPR-Cas Systems/*genetics/metabolism [MESH] |RNA-Binding Protein EWS/genetics/metabolism [MESH] |Recombinational DNA Repair [MESH] |Translocation, Genetic [MESH]Generation of chromosomal translocations that lead to conditional fusi(epmc).pdf DeepDyve Pubget Overpricing