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10.1136/gutjnl-2015-310016 http://scihub22266oqcxt.onion/10.1136/gutjnl-2015-310016 C5529969!5529969!26719303     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Gut 2017 ; 66 (4): 692-704 Nephropedia Template TP {{tp|p=26719303|t=2017. Dual role of tumour-infiltrating T helper 17 cells in human colorectal cancer |pdf=|usr=}}{{26719303}} gab.com Text Dual role of tumour-infiltrating T helper 17 cells in human colorectal cancer JO: Gut http://www.kidney.de/pget.php?&tw=1&pmid=26719303 #FOAvip Background: The immune contexture predicts prognosis in human colorectal cancer (CRC). Whereas tumour-infiltrating CD8+ T cells and myeloid CD16+ myeloperoxidase (MPO)+ cells are associated with favourable clinical outcome, interleukin (IL)-17-producing cells have been reported to correlate with severe prognosis. However, their phenotypes and functions continue to be debated. Objective: To investigate clinical relevance, phenotypes and functional features of CRC-infiltrating, IL-17-producing cells. Methods: IL-17 staining was performed by immunohistochemistry on a tissue microarray including 1148 CRCs. Phenotypes of IL-17-producing cells were evaluated by flow cytometry on cell suspensions obtained by enzymatic digestion of clinical specimens. Functions of CRC-isolated, IL-17-producing cells were assessed by in vitro and in vivo experiments. Results: IL-17+ infiltrates were not themselves predictive of an unfavourable clinical outcome, but correlated with infiltration by CD8+ T cells and CD16+ MPO+ neutrophils. Ex vivo analysis showed that tumour-infiltrating IL-17+ cells mostly consist of CD4+ T helper 17 (Th17) cells with multifaceted properties. Indeed, owing to IL-17 secretion, CRC-derived Th17 triggered the release of protumorigenic factors by tumour and tumour-associated stroma. However, on the other hand, they favoured recruitment of beneficial neutrophils through IL-8 secretion and, most importantly, they drove highly cytotoxic CCR5+CCR6+CD8+ T cells into tumour tissue, through CCL5 and CCL20 release. Consistent with these findings, the presence of intraepithelial, but not of stromal Th17 cells, positively correlated with improved survival. Conclusions: Our study shows the dual role played by tumour-infiltrating Th17 in CRC, thus advising caution when developing new IL-17/Th17 targeted treatments. Twit Text FOAVip Dual role of tumour-infiltrating T helper 17 cells in human colorectal cancer ????Gut http://www.kidney.de/pget.php?&tw=1&pmid=26719303 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26719303 #FOAvip English Wikipedia <ref>{{Cite pmid|26719303}}</ref> Dual role of tumour-infiltrating T helper 17 cells in human colorectal cancer #MMPMID26719303 Amicarella F; Muraro MG; Hirt C; Cremonesi E; Padovan E; Mele V; Governa V; Han J; Huber X; Droeser RA; Zuber M; Adamina M; Bolli M; Rosso R; Lugli A; Zlobec I; Terracciano L; Tornillo L; Zajac P; Eppenberger-Castori S; Trapani F; Oertli D; Iezzi G Gut 2017[Apr]; 66 (4): 692-704 PMID26719303show ga Background: The immune contexture predicts prognosis in human colorectal cancer (CRC). Whereas tumour-infiltrating CD8+ T cells and myeloid CD16+ myeloperoxidase (MPO)+ cells are associated with favourable clinical outcome, interleukin (IL)-17-producing cells have been reported to correlate with severe prognosis. However, their phenotypes and functions continue to be debated. Objective: To investigate clinical relevance, phenotypes and functional features of CRC-infiltrating, IL-17-producing cells. Methods: IL-17 staining was performed by immunohistochemistry on a tissue microarray including 1148 CRCs. Phenotypes of IL-17-producing cells were evaluated by flow cytometry on cell suspensions obtained by enzymatic digestion of clinical specimens. Functions of CRC-isolated, IL-17-producing cells were assessed by in vitro and in vivo experiments. Results: IL-17+ infiltrates were not themselves predictive of an unfavourable clinical outcome, but correlated with infiltration by CD8+ T cells and CD16+ MPO+ neutrophils. Ex vivo analysis showed that tumour-infiltrating IL-17+ cells mostly consist of CD4+ T helper 17 (Th17) cells with multifaceted properties. Indeed, owing to IL-17 secretion, CRC-derived Th17 triggered the release of protumorigenic factors by tumour and tumour-associated stroma. However, on the other hand, they favoured recruitment of beneficial neutrophils through IL-8 secretion and, most importantly, they drove highly cytotoxic CCR5+CCR6+CD8+ T cells into tumour tissue, through CCL5 and CCL20 release. Consistent with these findings, the presence of intraepithelial, but not of stromal Th17 cells, positively correlated with improved survival. Conclusions: Our study shows the dual role played by tumour-infiltrating Th17 in CRC, thus advising caution when developing new IL-17/Th17 targeted treatments. äDual role of tumour-infiltrating T helper 17 cells in human colorectal(epmc).pdf DeepDyve Pubget Overpricing