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10.1016/j.bpj.2017.06.014 http://scihub22266oqcxt.onion/10.1016/j.bpj.2017.06.014 C5529610!5529610 !28746857     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=28746857 &cmd=llinks): Failed to open stream: HTTP request failed! 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Enhancing Irreversible Electroporation by Manipulating Cellular Biophysics with a Molecular Adjuvant |pdf=|usr=}}{{28746857 }} gab.com Text Enhancing Irreversible Electroporation by Manipulating Cellular Biophysics with a Molecular Adjuvant JO: Biophys J http://www.kidney.de/pget.php?&tw=1&pmid=28746857 #FOAvip Pulsed electric fields applied to cells have been used as an invaluable research tool to enhance delivery of genes or other intracellular cargo, as well as for tumor treatment via electrochemotherapy or tissue ablation. These processes involve the buildup of charge across the cell membrane, with subsequent alteration of transmembrane potential that is a function of cell biophysics and geometry. For traditional electroporation parameters, larger cells experience a greater degree of membrane potential alteration. However, we have recently demonstrated that the nuclear/cytoplasm ratio (NCR), rather than cell size, is a key predictor of response for cells treated with high-frequency irreversible electroporation (IRE). In this study, we leverage a targeted molecular therapy, ephrinA1, known to markedly collapse the cytoplasm of cells expressing the EphA2 receptor, to investigate how biophysical cellular changes resulting from NCR manipulation affect the response to IRE at varying frequencies. We present evidence that the increase in the NCR mitigates the cell death response to conventional electroporation pulsed-electric fields (?100 ?s), consistent with the previously noted size dependence. However, this same molecular treatment enhanced the cell death response to high-frequency electric fields (?1 ?s). This finding demonstrates the importance of considering cellular biophysics and frequency-dependent effects in developing electroporation protocols, and our approach provides, to our knowledge, a novel and direct experimental methodology to quantify the relationship between cell morphology, pulse frequency, and electroporation response. Finally, this novel, to our knowledge, combinatorial approach may provide a paradigm to enhance in vivo tumor ablation through a molecular manipulation of cellular morphology before IRE application. Twit Text FOAVip Enhancing Irreversible Electroporation by Manipulating Cellular Biophysics with a Molecular Adjuvant ????Biophys J http://www.kidney.de/pget.php?&tw=1&pmid=28746857 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28746857 #FOAvip English Wikipedia <ref>{{Cite pmid|28746857 }}</ref> Enhancing Irreversible Electroporation by Manipulating Cellular Biophysics with a Molecular Adjuvant #MMPMID28746857 Ivey JW ; Latouche EL ; Richards ML ; Lesser GJ ; Debinski W ; Davalos RV ; Verbridge SS Biophys J 2017[Jul]; 113 (2 ): 472-480 PMID28746857 show ga Pulsed electric fields applied to cells have been used as an invaluable research tool to enhance delivery of genes or other intracellular cargo, as well as for tumor treatment via electrochemotherapy or tissue ablation. These processes involve the buildup of charge across the cell membrane, with subsequent alteration of transmembrane potential that is a function of cell biophysics and geometry. For traditional electroporation parameters, larger cells experience a greater degree of membrane potential alteration. However, we have recently demonstrated that the nuclear/cytoplasm ratio (NCR), rather than cell size, is a key predictor of response for cells treated with high-frequency irreversible electroporation (IRE). In this study, we leverage a targeted molecular therapy, ephrinA1, known to markedly collapse the cytoplasm of cells expressing the EphA2 receptor, to investigate how biophysical cellular changes resulting from NCR manipulation affect the response to IRE at varying frequencies. We present evidence that the increase in the NCR mitigates the cell death response to conventional electroporation pulsed-electric fields (?100 ?s), consistent with the previously noted size dependence. However, this same molecular treatment enhanced the cell death response to high-frequency electric fields (?1 ?s). This finding demonstrates the importance of considering cellular biophysics and frequency-dependent effects in developing electroporation protocols, and our approach provides, to our knowledge, a novel and direct experimental methodology to quantify the relationship between cell morphology, pulse frequency, and electroporation response. Finally, this novel, to our knowledge, combinatorial approach may provide a paradigm to enhance in vivo tumor ablation through a molecular manipulation of cellular morphology before IRE application. |Animals [MESH] |Astrocytes/drug effects/pathology [MESH] |Biomechanical Phenomena [MESH] |Cell Death/drug effects [MESH] |Cell Line, Tumor [MESH] |Cell Size [MESH] |Coculture Techniques [MESH] |Collagen [MESH] |Electromagnetic Fields [MESH] |Electroporation/*methods [MESH] |Ephrin-A1/*pharmacology [MESH] |Finite Element Analysis [MESH] |Glioma/drug therapy/pathology/therapy [MESH] |Humans [MESH] |Hydrogels [MESH] |Membrane Potentials [MESH] |Models, Biological [MESH] |Molecular Targeted Therapy/*methods [MESH] |Rats [MESH]Enhancing Irreversible Electroporation by Manipulating Cellular Biophy(epmc).pdf DeepDyve Pubget Overpricing