Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=28737476
&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests
in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215
A high-fidelity method for genomic sequencing of single somatic cells reveals a
very high mutational burden
#MMPMID28737476
Vijg J
; Dong X
; Zhang L
Exp Biol Med (Maywood)
2017[Jul]; 242
(13
): 1318-1324
PMID28737476
show ga
Postzygotic mutations in somatic cells lead to genome mosaicism and can be the
cause of cancer, possibly other human diseases and aging. Somatic mutations are
difficult to detect in bulk tissue samples. Here, we review the available assays
for measuring somatic mutations, with a focus on recent single-cell, whole genome
sequencing methods. Impact statement Somatic mutations cause cancer, possibly
other diseases and aging. Yet, very little is known about the frequency of such
mutations in vivo, their distribution across the genome, and their possible
functional consequences other than cancer. Even in cancer, we do not know the
heterogeneity of mutations within a tumor and if seemingly normal cells in its
surroundings already have elevated mutation frequencies. Here, we review a new,
whole genome amplification system that allows accurate quantification and
characterization of single-cell mutational landscapes in human cells and tissues
in relation to disease.
free
free
free
