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10.5487/TR.2017.33.3.211 http://scihub22266oqcxt.onion/10.5487/TR.2017.33.3.211 C5523555!5523555 !28744352     free     free     free Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 251.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 251.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 251.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 251.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 251.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 251.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 251.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\28744352 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Toxicol+Res 2017 ; 33 (3 ): 211-218 Nephropedia Template TP {{tp|p=28744352 |t=2017. CYP1B1 Activates Wnt/?-Catenin Signaling through Suppression of Herc5-Mediated ISGylation for Protein Degradation on ?-Catenin in HeLa Cells |pdf=|usr=}}{{28744352 }} gab.com Text CYP1B1 Activates Wnt/ -Catenin Signaling through Suppression of Herc5-Mediated ISGylation for Protein Degradation on -Catenin in HeLa Cells JO: Toxicol Res http://www.kidney.de/pget.php?&tw=1&pmid=28744352 #FOAvip Cytochrome P450 1B1 (CYP1B1) acts as a hydroxylase for estrogen and activates potential carcinogens. Moreover, its expression in tumor tissues is much higher than that in normal tissues. Despite this association between CYP1B1 and cancer, the detailed molecular mechanism of CYP1B1 on cancer progression in HeLa cells remains unknown. Previous reports indicated that the mRNA expression level of Herc5, an E3 ligase for ISGylation, is promoted by CYP1B1 suppression using specific small interfering RNA, and that ISGylation may be involved in ubiquitination related to ?-catenin degradation. With this background, we investigated the relationships among CYP1B1, Herc5, and ?-catenin. RT-PCR and western blot analyses showed that CYP1B1 overexpression induced and CYP1B1 inhibition reduced, respectively, the expression of Wnt/?-catenin signaling target genes including ?-catenin and cyclin D1. Moreover, HeLa cells were treated with the CYP1B1 inducer 7,12-dimethylbenz[?]anthracene (DMBA) or the CYP1B1 specific inhibitor, tetramethoxystilbene (TMS) and consequently DMBA increased and TMS decreased ?-catenin and cyclin D1 expression, respectively. To determine the correlation between CYP1B1 expression and ISGylation, the expression of ISG15, a ubiquitin-like protein, was detected following CYP1B1 regulation, which revealed that CYP1B1 may inhibit ISGylation through suppression of ISG15 expression. In addition, the mRNA and protein expression levels of Herc5 were strongly suppressed by CYP1B1. Finally, an immunoprecipitation assay revealed a direct physical interaction between Herc5 and ?-catenin in HeLa cells. In conclusion, these data suggest that CYP1B1 may activate Wnt/?-catenin signaling through stabilization of ?-catenin protein from Herc5-mediated ISGylation for proteosomal degradation. Twit Text FOAVip CYP1B1 Activates Wnt/ -Catenin Signaling through Suppression of Herc5-Mediated ISGylation for Protein Degradation on -Catenin in HeLa Cells ????Toxicol Res http://www.kidney.de/pget.php?&tw=1&pmid=28744352 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28744352 #FOAvip English Wikipedia <ref>{{Cite pmid|28744352 }}</ref> CYP1B1 Activates Wnt/?-Catenin Signaling through Suppression of Herc5-Mediated ISGylation for Protein Degradation on ?-Catenin in HeLa Cells #MMPMID28744352 Park YS ; Kwon YJ ; Chun YJ Toxicol Res 2017[Jul]; 33 (3 ): 211-218 PMID28744352 show ga Cytochrome P450 1B1 (CYP1B1) acts as a hydroxylase for estrogen and activates potential carcinogens. Moreover, its expression in tumor tissues is much higher than that in normal tissues. Despite this association between CYP1B1 and cancer, the detailed molecular mechanism of CYP1B1 on cancer progression in HeLa cells remains unknown. Previous reports indicated that the mRNA expression level of Herc5, an E3 ligase for ISGylation, is promoted by CYP1B1 suppression using specific small interfering RNA, and that ISGylation may be involved in ubiquitination related to ?-catenin degradation. With this background, we investigated the relationships among CYP1B1, Herc5, and ?-catenin. RT-PCR and western blot analyses showed that CYP1B1 overexpression induced and CYP1B1 inhibition reduced, respectively, the expression of Wnt/?-catenin signaling target genes including ?-catenin and cyclin D1. Moreover, HeLa cells were treated with the CYP1B1 inducer 7,12-dimethylbenz[?]anthracene (DMBA) or the CYP1B1 specific inhibitor, tetramethoxystilbene (TMS) and consequently DMBA increased and TMS decreased ?-catenin and cyclin D1 expression, respectively. To determine the correlation between CYP1B1 expression and ISGylation, the expression of ISG15, a ubiquitin-like protein, was detected following CYP1B1 regulation, which revealed that CYP1B1 may inhibit ISGylation through suppression of ISG15 expression. In addition, the mRNA and protein expression levels of Herc5 were strongly suppressed by CYP1B1. Finally, an immunoprecipitation assay revealed a direct physical interaction between Herc5 and ?-catenin in HeLa cells. In conclusion, these data suggest that CYP1B1 may activate Wnt/?-catenin signaling through stabilization of ?-catenin protein from Herc5-mediated ISGylation for proteosomal degradation. äCYP1B1 Activates Wnt/?-Catenin Signaling through Suppression of Herc5-(epmc).pdf DeepDyve Pubget Overpricing