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Two Sets of Piwi Proteins Are Involved in Distinct sRNA Pathways Leading to
Elimination of Germline-Specific DNA
#MMPMID28700949
Furrer DI
; Swart EC
; Kraft MF
; Sandoval PY
; Nowacki M
Cell Rep
2017[Jul]; 20
(2
): 505-520
PMID28700949
show ga
Piwi proteins and piRNAs protect eukaryotic germlines against the spread of
transposons. During development in the ciliate Paramecium, two Piwi-dependent
sRNA classes are involved in the elimination of transposons and
transposon-derived DNA: scan RNAs (scnRNAs), associated with Ptiwi01 and Ptiwi09,
and iesRNAs, whose binding partners we now identify as Ptiwi10 and Ptiwi11.
scnRNAs derive from the maternal genome and initiate DNA elimination during
development, whereas iesRNAs continue DNA targeting until the removal process is
complete. Here, we show that scnRNAs and iesRNAs are processed by distinct
Dicer-like proteins and bind Piwi proteins in a mutually exclusive manner,
suggesting separate biogenesis pathways. We also demonstrate that the PTIWI10
gene is transcribed from the developing nucleus and that its transcription
depends on prior DNA excision, suggesting a mechanism of gene expression control
triggered by the removal of short DNA segments interrupting the gene.
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