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10.3390/ma6072978

http://scihub22266oqcxt.onion/10.3390/ma6072978
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suck abstract from ncbi


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pmid28811417
      Materials+(Basel) 2013 ; 6 (7 ): 2978-2988
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  • In Vivo Osteogenic Differentiation of Human Embryoid Bodies in an Injectable in Situ-Forming Hydrogel #MMPMID28811417
  • Kim DY ; Kim YY ; Lee HB ; Moon SY ; Ku SY ; Kim MS
  • Materials (Basel) 2013[Jul]; 6 (7 ): 2978-2988 PMID28811417 show ga
  • In this study, we examined the in vivo osteogenic differentiation of human embryoid bodies (hEBs) by using an injectable in situ-forming hydrogel. A solution containing MPEG-b-(polycaprolactone-ran-polylactide) (MCL) and hEBs was easily prepared at room temperature. The MCL solution with hEBs and osteogenic factors was injected into nude mice and developed into in situ-forming hydrogels at the injection sites; these hydrogels maintained their shape even after 12 weeks in vivo, thereby indicating that the in situ-forming MCL hydrogel was a suitable scaffold for hEBs. The in vivo osteogenic differentiation was observed only in the in situ gel-forming MCL hydrogel in the presence of hEBs and osteogenic factors. In conclusion, this preliminary study suggests that hEBs and osteogenic factors embedded in an in situ-forming MCL hydrogel may provide numerous benefits as a noninvasive alternative for allogeneic tissue engineering applications.
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