In Vivo Osteogenic Differentiation of Human Embryoid Bodies in an Injectable in
Situ-Forming Hydrogel
#MMPMID28811417
Kim DY
; Kim YY
; Lee HB
; Moon SY
; Ku SY
; Kim MS
Materials (Basel)
2013[Jul]; 6
(7
): 2978-2988
PMID28811417
show ga
In this study, we examined the in vivo osteogenic differentiation of human
embryoid bodies (hEBs) by using an injectable in situ-forming hydrogel. A
solution containing MPEG-b-(polycaprolactone-ran-polylactide) (MCL) and hEBs was
easily prepared at room temperature. The MCL solution with hEBs and osteogenic
factors was injected into nude mice and developed into in situ-forming hydrogels
at the injection sites; these hydrogels maintained their shape even after 12
weeks in vivo, thereby indicating that the in situ-forming MCL hydrogel was a
suitable scaffold for hEBs. The in vivo osteogenic differentiation was observed
only in the in situ gel-forming MCL hydrogel in the presence of hEBs and
osteogenic factors. In conclusion, this preliminary study suggests that hEBs and
osteogenic factors embedded in an in situ-forming MCL hydrogel may provide
numerous benefits as a noninvasive alternative for allogeneic tissue engineering
applications.