Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\28534411
.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117 Nano+Lett
2017 ; 17
(6
): 3932-3939
Nephropedia Template TP
gab.com Text
Twit Text FOAVip
Twit Text #
English Wikipedia
Intracellular and Extracellular Recording of Spontaneous Action Potentials in
Mammalian Neurons and Cardiac Cells with 3D Plasmonic Nanoelectrodes
#MMPMID28534411
Dipalo M
; Amin H
; Lovato L
; Moia F
; Caprettini V
; Messina GC
; Tantussi F
; Berdondini L
; De Angelis F
Nano Lett
2017[Jun]; 17
(6
): 3932-3939
PMID28534411
show ga
Three-dimensional vertical micro- and nanostructures can enhance the signal
quality of multielectrode arrays and promise to become the prime methodology for
the investigation of large networks of electrogenic cells. So far, access to the
intracellular environment has been obtained via spontaneous poration,
electroporation, or by surface functionalization of the micro/nanostructures;
however, these methods still suffer from some limitations due to their intrinsic
characteristics that limit their widespread use. Here, we demonstrate the ability
to continuously record both extracellular and intracellular-like action
potentials at each electrode site in spontaneously active mammalian neurons and
HL-1 cardiac-derived cells via the combination of vertical nanoelectrodes with
plasmonic optoporation. We demonstrate long-term and stable recordings with a
very good signal-to-noise ratio. Additionally, plasmonic optoporation does not
perturb the spontaneous electrical activity; it permits continuous recording even
during the poration process and can regulate extracellular and intracellular
contributions by means of partial cellular poration.