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10.1038/s41598-017-06325-6

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C5519553!5519553!28729645
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suck abstract from ncbi


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pmid28729645      Sci+Rep 2017 ; 7 (ä): ä
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  • Nano-antenna enhanced two-focus fluorescence correlation spectroscopy #MMPMID28729645
  • Langguth L; Szuba A; Mann SA; Garnett EC; Koenderink GH; Koenderink AF
  • Sci Rep 2017[]; 7 (ä): ä PMID28729645show ga
  • We propose two-focus fluorescence correlation spectroscopy (2fFCS) on basis of plasmonic nanoantennas that provide distinct hot spots that are individually addressable through polarization, yet lie within a single diffraction limited microscope focus. The importance of two-focus FCS is that a calibrated distance between foci provides an intrinsic calibration to derive diffusion constants from measured correlation times. Through electromagnetic modelling we analyze a geometry of perpendicular nanorods, and their inverse, i.e., nanoslits. While we find that nanorods are not suited for nano-antenna enhanced 2fFCS due to substantial background signal, a nanoslit geometry is expected to provide a di tinct cross-correlation between orthogonally polarized detection channels. Furthermore, by utilizing a periodic array of nanoslits instead of a single pair, the amplitude of the cross-correlation can be enhanced. To demonstrate this technique, we present a proof of principle experiment on the basis of a periodic array of nanoslits, applied to lipid diffusion in a supported lipid bilayer.
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