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Aptasensor with Expanded Nucleotide Using DNA Nanotetrahedra for Electrochemical
Detection of Cancerous Exosomes
#MMPMID28287705
Wang S
; Zhang L
; Wan S
; Cansiz S
; Cui C
; Liu Y
; Cai R
; Hong C
; Teng IT
; Shi M
; Wu Y
; Dong Y
; Tan W
ACS Nano
2017[Apr]; 11
(4
): 3943-3949
PMID28287705
show ga
Exosomes are extracellular vesicles (50-100 nm) circulating in biofluids as
intercellular signal transmitters. Although the potential of cancerous exosomes
as tumor biomarkers is promising, sensitive and rapid detection of exosomes
remains challenging. Herein, we combined the strengths of advanced aptamer
technology, DNA-based nanostructure, and portable electrochemical devices to
develop a nanotetrahedron (NTH)-assisted aptasensor for direct capture and
detection of hepatocellular exosomes. The oriented immobilization of aptamers
significantly improved the accessibility of an artificial nucleobase-containing
aptamer to suspended exosomes, and the NTH-assisted aptasensor could detect
exosomes with 100-fold higher sensitivity when compared to the single-stranded
aptamer-functionalized aptasensor. The present study provides a proof-of-concept
for sensitive and efficient quantification of tumor-derived exosomes. We thus
expect the NTH-assisted electrochemical aptasensor to become a powerful tool for
comprehensive exosome studies.
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