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Antimicrobial Efficacy and Synergy of Metal Ions against Enterococcus faecium,
Klebsiella pneumoniae and Acinetobacter baumannii in Planktonic and Biofilm
Phenotypes
#MMPMID28724953
Vaidya MY
; McBain AJ
; Butler JA
; Banks CE
; Whitehead KA
Sci Rep
2017[Jul]; 7
(1
): 5911
PMID28724953
show ga
The effects of metal ion solutions (silver, copper, platinum, gold and palladium)
were determined individually and in combination against Enterococcus faecium,
Acinetobacter baumannii and Klebsiella pneumoniae. Platinum, gold and palladium
showed the greatest antimicrobial efficacy in zone of inhibition (ZoI) assays.
When tested in combinations using ZoI assays, gold/platinum, gold/palladium and
platinum/palladium were indicative of synergy. Microbial inhibitory concentration
demonstrated platinum and gold against Enterococcus faecium, platinum against
Klebsiella pneumoniae and platinum and silver against Acinetobacter baumannii
were optimal. Minimal bactericidal concentrations determined the greatest
bactericidal activity was again platinum gold and palladium against all three
bacteria. Fractional Inhibitory Concentration (FIC) studies demonstrated that the
silver/platinum combination against Enterococcus faecium, and silver/copper
combination against Acinetobacter baumannii demonstrated antimicrobial synergy.
Following crystal violet biofilm assays for single metal ion solutions,
antimicrobial efficacies were demonstrated for all the metals against all the
bacteria Synergistic assays against biofilms demonstrated gold/palladium,
gold/platinumand platinum/palladium resulted in the greatest antimicrobial
efficacy. Overall, platinum, palladium and gold metal ion solutions in individual
use or combination demonstrated the greatest antimicrobial efficacies against
planktonic or biofilm bacteria. This work demonstrates the potential for using a
range of metal ions, as biocidal formulations against both planktonic or biofilm
bacteria.
|Acinetobacter baumannii/*drug effects
[MESH]
|Anti-Infective Agents/*pharmacology
[MESH]
|Biofilms/*drug effects/growth & development
[MESH]