Dual functionality nanobioconjugates targeting intracellular bacteria in cancer
cells with enhanced antimicrobial activity
#MMPMID28724927
Singh R
; Patil S
; Singh N
; Gupta S
Sci Rep
2017[Jul]; 7
(1
): 5792
PMID28724927
show ga
Bacterial drug resistance has emerged as a serious global threat mandating the
development of novel methodologies that allow facile modulation of antimicrobial
action in a controlled fashion. Conjugating antibiotics to nanoparticles helps to
meet this goal by increasing the drug's overall avidity, bioavailability and
easier internalisation into mammalian cells, targeting bacteria that otherwise
escape antibacterial action by host cell-localisation. We used polymyxin B
sulfate (PMB) and sushi peptide as model drugs against Gram-negative bacteria and
established their enhanced antimicrobial activity on Escherichia coli (E. coli)
cells after conjugation to gold nanoparticles (AuNPs). The efficacy of the
bioconjugates was also tested on Salmonella typhi (S. typhi) bacteria infected
into cervical cancer cells (HeLa) and further improved through specific targeting
via folate receptors. Our results demonstrate significantly lower inhibitory
concentration values for sushi-NP assemblies as compared to free drug, especially
at optimal drug loading levels. No major cytotoxicity was observed in mammalian
cells alone.
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