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2017 ; 586
(ä): 359-378
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Recent Achievements in Characterizing the Histone Code and Approaches to
Integrating Epigenomics and Systems Biology
#MMPMID28137571
Janssen KA
; Sidoli S
; Garcia BA
Methods Enzymol
2017[]; 586
(ä): 359-378
PMID28137571
show ga
Functional epigenetic regulation occurs by dynamic modification of chromatin,
including genetic material (i.e., DNA methylation), histone proteins, and other
nuclear proteins. Due to the highly complex nature of the histone code, mass
spectrometry (MS) has become the leading technique in identification of single
and combinatorial histone modifications. MS has now overcome antibody-based
strategies due to its automation, high resolution, and accurate quantitation.
Moreover, multiple approaches to analysis have been developed for global
quantitation of posttranslational modifications (PTMs), including large-scale
characterization of modification coexistence (middle-down and top-down
proteomics), which is not currently possible with any other biochemical strategy.
Recently, our group and others have simplified and increased the effectiveness of
analyzing histone PTMs by improving multiple MS methods and data analysis tools.
This review provides an overview of the major achievements in the analysis of
histone PTMs using MS with a focus on the most recent improvements. We speculate
that the workflow for histone analysis at its state of the art is highly reliable
in terms of identification and quantitation accuracy, and it has the potential to
become a routine method for systems biology thanks to the possibility of
integrating histone MS results with genomics and proteomics datasets.
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