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10.1016/j.stemcr.2017.04.026 http://scihub22266oqcxt.onion/10.1016/j.stemcr.2017.04.026 C5511042!5511042!28552604     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Stem+Cell+Reports 2017 ; 9 (1): 279-91 Nephropedia Template TP {{tp|p=28552604|t=2017. Evidence of In Vitro Preservation of Human Nephrogenesis at the Single-Cell Level |pdf=|usr=}}{{28552604}} gab.com Text Evidence of In Vitro Preservation of Human Nephrogenesis at the Single-Cell Level JO: Stem Cell Reports http://www.kidney.de/pget.php?&tw=1&pmid=28552604 #FOAvip During nephrogenesis, stem/progenitor cells differentiate and give rise to early nephron structures that segment to proximal and distal nephron cell types. Previously, we prospectively isolated progenitors from human fetal kidney (hFK) utilizing a combination of surface markers. However, upon culture nephron progenitors differentiated and could not be robustly maintained in vitro. Here, by culturing hFK in a modified medium used for in vitro growth of mouse nephron progenitors, and by dissection of NCAM+/CD133? progenitor cells according to EpCAM expression (NCAM+/CD133?/EpCAM?, NCAM+/CD133?/EpCAMdim, NCAM+/CD133?/EpCAMbright), we show at single-cell resolution a preservation of uninduced and induced cap mesenchyme as well as a transitioning mesenchymal-epithelial state. Concomitantly, differentiating and differentiated epithelial lineages are also maintained. In vitro expansion of discrete stages of early human nephrogenesis in nephron stem cell cultures may be used for drug screening on a full repertoire of developing kidney cells and for prospective isolation of mesenchymal or epithelial renal lineages for regenerative medicine. Twit Text FOAVip Evidence of In Vitro Preservation of Human Nephrogenesis at the Single-Cell Level ????Stem Cell Reports http://www.kidney.de/pget.php?&tw=1&pmid=28552604 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28552604 #FOAvip English Wikipedia <ref>{{Cite pmid|28552604}}</ref> Evidence of In Vitro Preservation of Human Nephrogenesis at the Single-Cell Level #MMPMID28552604 Pode-Shakked N; Gershon R; Tam G; Omer D; Gnatek Y; Kanter I; Oriel S; Katz G; Harari-Steinberg O; Kalisky T; Dekel B Stem Cell Reports 2017[Jul]; 9 (1): 279-91 PMID28552604show ga During nephrogenesis, stem/progenitor cells differentiate and give rise to early nephron structures that segment to proximal and distal nephron cell types. Previously, we prospectively isolated progenitors from human fetal kidney (hFK) utilizing a combination of surface markers. However, upon culture nephron progenitors differentiated and could not be robustly maintained in vitro. Here, by culturing hFK in a modified medium used for in vitro growth of mouse nephron progenitors, and by dissection of NCAM+/CD133? progenitor cells according to EpCAM expression (NCAM+/CD133?/EpCAM?, NCAM+/CD133?/EpCAMdim, NCAM+/CD133?/EpCAMbright), we show at single-cell resolution a preservation of uninduced and induced cap mesenchyme as well as a transitioning mesenchymal-epithelial state. Concomitantly, differentiating and differentiated epithelial lineages are also maintained. In vitro expansion of discrete stages of early human nephrogenesis in nephron stem cell cultures may be used for drug screening on a full repertoire of developing kidney cells and for prospective isolation of mesenchymal or epithelial renal lineages for regenerative medicine. äEvidence of In Vitro Preservation of Human Nephrogenesis at the Single(epmc).pdf DeepDyve Pubget Overpricing