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10.1038/nmeth.4235

http://scihub22266oqcxt.onion/10.1038/nmeth.4235
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C5505470!5505470!28346450
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suck abstract from ncbi


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pmid28346450      Nat+Methods 2017 ; 14 (5): 487-90
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  • Structural modeling of protein-RNA complexes using crosslinking of segmentally isotope labeled RNA and MS/MS #MMPMID28346450
  • Dorn G; Leitner A; Boudet J; Campagne S; von Schroetter C; Moursy A; Aebersold R; Allain FHT
  • Nat Methods 2017[May]; 14 (5): 487-90 PMID28346450show ga
  • Ribonucleoproteins (RNPs) are key regulators of cellular function. We established an efficient approach that combines segmental isotope labeling of RNA with photo-crosslinking and tandem mass spectrometry to localize protein-RNA interactions simultaneously at amino acid and nucleotide resolution. The approach was tested on Polypyrimidine Tract Binding Protein 1 and U1 small nuclear RNP and the results support integrative atomic-scale structural modeling thus providing mechanistic insights into RNP regulated processes.
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