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10.3892/ijmm.2017.3016 http://scihub22266oqcxt.onion/10.3892/ijmm.2017.3016 C5505014!5505014 !28627592     free     free     free Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 251.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 251.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 251.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 251.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 251.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 284.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 284.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 284.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 284.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\28627592 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Int+J+Mol+Med 2017 ; 40 (2 ): 337-346 Nephropedia Template TP {{tp|p=28627592 |t=2017. Triptolide exerts protective effects against fibrosis following ileocolonic anastomosis by mechanisms involving the miR-16-1/HSP70 pathway in IL-10-deficient mice |pdf=|usr=}}{{28627592 }} gab.com Text Triptolide exerts protective effects against fibrosis following ileocolonic anastomosis by mechanisms involving the miR-16-1/HSP70 pathway in IL-10-deficient mice JO: Int J Mol Med http://www.kidney.de/pget.php?&tw=1&pmid=28627592 #FOAvip Surgeries, particularly ileocecal resection (ICR), are often required in the treatment of Crohn's disease (CD). However, recurrences are common for patients who undergo ICR, and anastomotic fibrosis is the main cause of re-operation. The present study aimed to investigate the therapeutic effects of triptolide (TPL) in ameliorating fibrosis following ileocolonic anastomosis. A model of IL?10?/? mice undergoing ICR was used to study postsurgical inflammation and fibrosis of anastomosis. For this purpsose, interleukin (IL)?10?/? mice were randomly divided into 3 groups as follows: the control group, the saline?treated group subjected to ICR (ST?ICR) and the TPL?treated group subjected to ICR (TT?ICR). Wild?type (WT) mice of matching ages were assigned to the WT group. The effects of TPL treatment on ileocolonic anastomosis were determined by histopathological evaluation, western blot analysis and ELISA. The analysis of the effects of TPL treatment on microRNA?16?1 (miR?16?1) and heat shock protein 70 (HSP70) expression was carried out by RT?qPCR and western blot analysis. Compared with the control group, significantly higher inflammation scores following anastomosis were observed in the ST?ICR group (P<0.05), although reversion was observed in the TT?ICR group, which was consistent with changes in the area of CD4+ cell infiltration. The elevated fibrosis scores and the overexpression of procollagen I and III in the ST?ICR group were all inhibited by TPL. With an increase in the severity of inflammation and fibrosis, the levels of IL?6, tumor necrosis factor?? (TNF??) and transforming growth factor??1 (TGF??1) increased; however, a significant decrease in these levels was observed following treatment with TPL (P<0.05). The results of RT?qPCR revealed that the upregulated miR?16?1 levels in the ST?ICR group were significantly reduced by TPL. HSP70, which can be inhibited by miR-16-1, ameliorates anastomotic inflammation and fibrosis. Thus, the present study demonstrates that TPL exerts a protective effect against fibrosis following anastomosis in CD. The miR?16?1/HSP70 signaling pathway, which can be regulated by TPL, may thus represent a novel therapeutic option in CD that deserves further investigation. Twit Text FOAVip Triptolide exerts protective effects against fibrosis following ileocolonic anastomosis by mechanisms involving the miR-16-1/HSP70 pathway in IL-10-deficient mice ????Int J Mol Med http://www.kidney.de/pget.php?&tw=1&pmid=28627592 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28627592 #FOAvip English Wikipedia <ref>{{Cite pmid|28627592 }}</ref> Triptolide exerts protective effects against fibrosis following ileocolonic anastomosis by mechanisms involving the miR-16-1/HSP70 pathway in IL-10-deficient mice #MMPMID28627592 Hou HW ; Wang JM ; Wang D ; Wu R ; Ji ZL Int J Mol Med 2017[Aug]; 40 (2 ): 337-346 PMID28627592 show ga Surgeries, particularly ileocecal resection (ICR), are often required in the treatment of Crohn's disease (CD). However, recurrences are common for patients who undergo ICR, and anastomotic fibrosis is the main cause of re-operation. The present study aimed to investigate the therapeutic effects of triptolide (TPL) in ameliorating fibrosis following ileocolonic anastomosis. A model of IL?10?/? mice undergoing ICR was used to study postsurgical inflammation and fibrosis of anastomosis. For this purpsose, interleukin (IL)?10?/? mice were randomly divided into 3 groups as follows: the control group, the saline?treated group subjected to ICR (ST?ICR) and the TPL?treated group subjected to ICR (TT?ICR). Wild?type (WT) mice of matching ages were assigned to the WT group. The effects of TPL treatment on ileocolonic anastomosis were determined by histopathological evaluation, western blot analysis and ELISA. The analysis of the effects of TPL treatment on microRNA?16?1 (miR?16?1) and heat shock protein 70 (HSP70) expression was carried out by RT?qPCR and western blot analysis. Compared with the control group, significantly higher inflammation scores following anastomosis were observed in the ST?ICR group (P<0.05), although reversion was observed in the TT?ICR group, which was consistent with changes in the area of CD4+ cell infiltration. The elevated fibrosis scores and the overexpression of procollagen I and III in the ST?ICR group were all inhibited by TPL. With an increase in the severity of inflammation and fibrosis, the levels of IL?6, tumor necrosis factor?? (TNF??) and transforming growth factor??1 (TGF??1) increased; however, a significant decrease in these levels was observed following treatment with TPL (P<0.05). The results of RT?qPCR revealed that the upregulated miR?16?1 levels in the ST?ICR group were significantly reduced by TPL. HSP70, which can be inhibited by miR-16-1, ameliorates anastomotic inflammation and fibrosis. Thus, the present study demonstrates that TPL exerts a protective effect against fibrosis following anastomosis in CD. The miR?16?1/HSP70 signaling pathway, which can be regulated by TPL, may thus represent a novel therapeutic option in CD that deserves further investigation. |Anastomosis, Surgical/*adverse effects/methods [MESH] |Animals [MESH] |Diterpenes/*therapeutic use [MESH] |Epoxy Compounds/therapeutic use [MESH] |Gene Deletion [MESH] |Gene Expression Regulation/drug effects [MESH] |HSP70 Heat-Shock Proteins/*genetics [MESH] |Inflammation/etiology/genetics/pathology/prevention & control [MESH] |Interleukin-10/*genetics [MESH] |Intestines/drug effects/*pathology/surgery [MESH] |Male [MESH] |Mice [MESH] |MicroRNAs/*genetics [MESH] |Phenanthrenes/*therapeutic use [MESH] |Postoperative Complications/genetics/pathology/*prevention & control [MESH] |Protective Agents/*therapeutic use [MESH]Triptolide exerts protective effects against fibrosis following ileoco(epmc).pdf DeepDyve Pubget Overpricing