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10.1021/acs.jproteome.7b00068 http://scihub22266oqcxt.onion/10.1021/acs.jproteome.7b00068 C5504490!5504490!28516784     free     free     free Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 261.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 261.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 261.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Proteome+Res 2017 ; 16 (7): 2457-71 Nephropedia Template TP {{tp|p=28516784|t=2017. In-Culture Cross-Linking of Bacterial Cells Reveals Large-Scale Dynamic Protein?Protein Interactions at the Peptide Level |pdf=|usr=}}{{28516784}} gab.com Text In-Culture Cross-Linking of Bacterial Cells Reveals Large-Scale Dynamic Protein Protein Interactions at the Peptide Level JO: J Proteome Res http://www.kidney.de/pget.php?&tw=1&pmid=28516784 #FOAvip Identification of dynamic protein?protein interactions at the peptide level on a proteomic scale is a challenging approach that is still in its infancy. We have developed a system to cross-link cells directly in culture with the special lysine cross-linker bis(succinimidyl)-3-azidomethyl-glutarate (BAMG). We used the Gram-positive model bacterium Bacillus subtilis as an exemplar system. Within 5 min extensive intracellular cross-linking was detected, while intracellular cross-linking in a Gram-negative species, Escherichia coli, was still undetectable after 30 min, in agreement with the low permeability in this organism for lipophilic compounds like BAMG. We were able to identify 82 unique interprotein cross-linked peptides with <1% false discovery rate by mass spectrometry and genome-wide database searching. Nearly 60% of the interprotein cross-links occur in assemblies involved in transcription and translation. Several of these interactions are new, and we identified a binding site between the ? and ?? subunit of RNA polymerase close to the downstream DNA channel, providing a clue into how ? might regulate promoter selectivity and promote RNA polymerase recycling. Our methodology opens new avenues to investigate the functional dynamic organization of complex protein assemblies involved in bacterial growth. Data are available via ProteomeXchange with identifier PXD006287. Twit Text FOAVip In-Culture Cross-Linking of Bacterial Cells Reveals Large-Scale Dynamic Protein Protein Interactions at the Peptide Level ????J Proteome Res http://www.kidney.de/pget.php?&tw=1&pmid=28516784 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28516784 #FOAvip English Wikipedia <ref>{{Cite pmid|28516784}}</ref> In-Culture Cross-Linking of Bacterial Cells Reveals Large-Scale Dynamic Protein?Protein Interactions at the Peptide Level #MMPMID28516784 de Jong L; de Koning EA; Roseboom W; Buncherd H; Wanner MJ; Dapic I; Jansen PJ; van Maarseveen JH; Corthals GL; Lewis PJ; Hamoen LW; de Koster CG J Proteome Res 2017[Jul]; 16 (7): 2457-71 PMID28516784show ga Identification of dynamic protein?protein interactions at the peptide level on a proteomic scale is a challenging approach that is still in its infancy. We have developed a system to cross-link cells directly in culture with the special lysine cross-linker bis(succinimidyl)-3-azidomethyl-glutarate (BAMG). We used the Gram-positive model bacterium Bacillus subtilis as an exemplar system. Within 5 min extensive intracellular cross-linking was detected, while intracellular cross-linking in a Gram-negative species, Escherichia coli, was still undetectable after 30 min, in agreement with the low permeability in this organism for lipophilic compounds like BAMG. We were able to identify 82 unique interprotein cross-linked peptides with <1% false discovery rate by mass spectrometry and genome-wide database searching. Nearly 60% of the interprotein cross-links occur in assemblies involved in transcription and translation. Several of these interactions are new, and we identified a binding site between the ? and ?? subunit of RNA polymerase close to the downstream DNA channel, providing a clue into how ? might regulate promoter selectivity and promote RNA polymerase recycling. Our methodology opens new avenues to investigate the functional dynamic organization of complex protein assemblies involved in bacterial growth. Data are available via ProteomeXchange with identifier PXD006287. äIn-Culture Cross-Linking of Bacterial Cells Reveals Large-Scale Dynami(epmc).pdf DeepDyve Pubget Overpricing