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2015 ; 6
(8
): 4884-4888
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English Wikipedia
HOCl can appear in the mitochondria of macrophages during bacterial infection as
revealed by a sensitive mitochondrial-targeting fluorescent probe
#MMPMID28717494
Zhou J
; Li L
; Shi W
; Gao X
; Li X
; Ma H
Chem Sci
2015[Aug]; 6
(8
): 4884-4888
PMID28717494
show ga
Macrophages, important cells of the innate immune system, can produce abundant
HOCl in the cytoplasm to fight against bacteria. Recent studies suggest that
mitochondria in macrophages play a role in antibacterial responses. During
bacterial infection, however, it is uncertain whether HOCl is present in the
mitochondria, mainly because of the lack of a suitable research method. Herein,
by developing a new mitochondrial-targeting fluorescent HOCl probe, combined with
confocal fluorescence imaging, we show for the first time that HOCl can appear in
the mitochondria of macrophages (Raw264.7 cells) during bacterial infection, as
confirmed with non-phagocytic cells and inhibitors as control experiments.
Moreover, the developed probe exhibits an accurate mitochondrial-targeting
ability, a fast response, and high selectivity and sensitivity (detection limit 9
nM), and is thus expected to be employed for further revealing the biological
function of subcellular mitochondria.