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10.1158/0008-5472.CAN-12-0735 http://scihub22266oqcxt.onion/10.1158/0008-5472.CAN-12-0735 C5500294!5500294 !23066032     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\23066032 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Cancer+Res 2013 ; 73 (3 ): 1076-85 Nephropedia Template TP {{tp|p=23066032 |t=2013. Methylome profiling reveals distinct alterations in phenotypic and mutational subgroups of myeloproliferative neoplasms |pdf=|usr=}}{{23066032 }} gab.com Text Methylome profiling reveals distinct alterations in phenotypic and mutational subgroups of myeloproliferative neoplasms JO: Cancer Res http://www.kidney.de/pget.php?&tw=1&pmid=23066032 #FOAvip Even though mutations in epigenetic regulators frequently occur in myeloproliferative neoplasms, their effects on the epigenome have not been well studied. Furthermore, even though primary myelofibrosis (PMF) has a markedly worse prognosis than essential thrombocytosis or polycythemia vera, the molecular distinctions between these subgroups are not well elucidated. We conducted the HELP (HpaII tiny fragment enriched by LM-PCR) assay to study genome-wide methylation in polycythemia vera, essential thrombocytosis, and PMF samples compared with healthy controls. We determined that polycythemia vera and essential thrombocytosis are characterized by aberrant promoter hypermethylation, whereas PMF is an epigenetically distinct subgroup characterized by both aberrant hyper- and hypomethylation. Aberrant hypomethylation in PMF was seen to occur in non-CpG island loci, showing further qualitative differences between the disease subgroups. The differentially methylated genes in polycythemia vera and essential thrombocytosis were involved predominantly in cell signaling pathways and were enriched for binding sites of GATA1 and other transcription factors. In contrast, aberrantly methylated genes in PMF were involved in inflammatory pathways and were enriched for NF1, LEF1, and other transcription factors. Within the PMF subgroup, cases with ASXL1 disruptions formed an epigenetically distinct subgroup with relatively increased methylation. Cases of myeloproliferative neoplasms (MPN) with TET2 mutations showed decreased levels of hydroxymethylation and distinct set of hypermethylated genes. In contrast, the JAK2V617F mutation did not drive epigenetic clustering within MPNs. Finally, the significance of aberrant methylation was shown by sensitivity of MPN-derived cell lines to decitabine. These results show epigenetic differences between PMF and polycythemia vera/essential thrombocytosis and reveal methylomic signatures of ASXL1 and TET2 mutations. Twit Text FOAVip Methylome profiling reveals distinct alterations in phenotypic and mutational subgroups of myeloproliferative neoplasms ????Cancer Res http://www.kidney.de/pget.php?&tw=1&pmid=23066032 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=23066032 #FOAvip English Wikipedia <ref>{{Cite pmid|23066032 }}</ref> Methylome profiling reveals distinct alterations in phenotypic and mutational subgroups of myeloproliferative neoplasms #MMPMID23066032 Nischal S ; Bhattacharyya S ; Christopeit M ; Yu Y ; Zhou L ; Bhagat TD ; Sohal D ; Will B ; Mo Y ; Suzuki M ; Pardanani A ; McDevitt M ; Maciejewski JP ; Melnick AM ; Greally JM ; Steidl U ; Moliterno A ; Verma A Cancer Res 2013[Feb]; 73 (3 ): 1076-85 PMID23066032 show ga Even though mutations in epigenetic regulators frequently occur in myeloproliferative neoplasms, their effects on the epigenome have not been well studied. Furthermore, even though primary myelofibrosis (PMF) has a markedly worse prognosis than essential thrombocytosis or polycythemia vera, the molecular distinctions between these subgroups are not well elucidated. We conducted the HELP (HpaII tiny fragment enriched by LM-PCR) assay to study genome-wide methylation in polycythemia vera, essential thrombocytosis, and PMF samples compared with healthy controls. We determined that polycythemia vera and essential thrombocytosis are characterized by aberrant promoter hypermethylation, whereas PMF is an epigenetically distinct subgroup characterized by both aberrant hyper- and hypomethylation. Aberrant hypomethylation in PMF was seen to occur in non-CpG island loci, showing further qualitative differences between the disease subgroups. The differentially methylated genes in polycythemia vera and essential thrombocytosis were involved predominantly in cell signaling pathways and were enriched for binding sites of GATA1 and other transcription factors. In contrast, aberrantly methylated genes in PMF were involved in inflammatory pathways and were enriched for NF1, LEF1, and other transcription factors. Within the PMF subgroup, cases with ASXL1 disruptions formed an epigenetically distinct subgroup with relatively increased methylation. Cases of myeloproliferative neoplasms (MPN) with TET2 mutations showed decreased levels of hydroxymethylation and distinct set of hypermethylated genes. In contrast, the JAK2V617F mutation did not drive epigenetic clustering within MPNs. Finally, the significance of aberrant methylation was shown by sensitivity of MPN-derived cell lines to decitabine. These results show epigenetic differences between PMF and polycythemia vera/essential thrombocytosis and reveal methylomic signatures of ASXL1 and TET2 mutations. |*DNA Methylation [MESH] |*Mutation [MESH] |Adult [MESH] |Aged [MESH] |Aged, 80 and over [MESH] |Azacitidine/analogs & derivatives/pharmacology [MESH] |Cell Line, Tumor [MESH] |DNA-Binding Proteins/genetics [MESH] |Decitabine [MESH] |Dioxygenases [MESH] |Female [MESH] |Humans [MESH] |Janus Kinase 2/genetics [MESH] |Male [MESH] |Middle Aged [MESH] |Polycythemia Vera/*genetics [MESH] |Primary Myelofibrosis/*genetics [MESH] |Proto-Oncogene Proteins/genetics [MESH] |Repressor Proteins/genetics [MESH]Methylome profiling reveals distinct alterations in phenotypic and mut(epmc).pdf DeepDyve Pubget Overpricing