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10.1093/nar/gkx292 http://scihub22266oqcxt.onion/10.1093/nar/gkx292 C5499584!5499584!28449067     free     free     free Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nucleic+Acids+Res 2017 ; 45 (12): e115 Nephropedia Template TP {{tp|p=28449067|t=2017. Using high-throughput barcode sequencing to efficiently map connectomes |pdf=|usr=}}{{28449067}} gab.com Text Using high-throughput barcode sequencing to efficiently map connectomes JO: Nucleic Acids Res http://www.kidney.de/pget.php?&tw=1&pmid=28449067 #FOAvip The function of a neural circuit is determined by the details of its synaptic connections. At present, the only available method for determining a neural wiring diagram with single synapse precision?a ?connectome??is based on imaging methods that are slow, labor-intensive and expensive. Here, we present SYNseq, a method for converting the connectome into a form that can exploit the speed and low cost of modern high-throughput DNA sequencing. In SYNseq, each neuron is labeled with a unique random nucleotide sequence?an RNA ?barcode??which is targeted to the synapse using engineered proteins. Barcodes in pre- and postsynaptic neurons are then associated through protein-protein crosslinking across the synapse, extracted from the tissue, and joined into a form suitable for sequencing. Although our failure to develop an efficient barcode joining scheme precludes the widespread application of this approach, we expect that with further development SYNseq will enable tracing of complex circuits at high speed and low cost. Twit Text FOAVip Using high-throughput barcode sequencing to efficiently map connectomes ????Nucleic Acids Res http://www.kidney.de/pget.php?&tw=1&pmid=28449067 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28449067 #FOAvip English Wikipedia <ref>{{Cite pmid|28449067}}</ref> Using high-throughput barcode sequencing to efficiently map connectomes #MMPMID28449067 Peikon ID; Kebschull JM; Vagin VV; Ravens DI; Sun YC; Brouzes E; Corrêa IR; Bressan D; Zador AM Nucleic Acids Res 2017[Jul]; 45 (12): e115 PMID28449067show ga The function of a neural circuit is determined by the details of its synaptic connections. At present, the only available method for determining a neural wiring diagram with single synapse precision?a ?connectome??is based on imaging methods that are slow, labor-intensive and expensive. Here, we present SYNseq, a method for converting the connectome into a form that can exploit the speed and low cost of modern high-throughput DNA sequencing. In SYNseq, each neuron is labeled with a unique random nucleotide sequence?an RNA ?barcode??which is targeted to the synapse using engineered proteins. Barcodes in pre- and postsynaptic neurons are then associated through protein-protein crosslinking across the synapse, extracted from the tissue, and joined into a form suitable for sequencing. Although our failure to develop an efficient barcode joining scheme precludes the widespread application of this approach, we expect that with further development SYNseq will enable tracing of complex circuits at high speed and low cost. äUsing high-throughput barcode sequencing to efficiently map connectome(epmc).pdf DeepDyve Pubget Overpricing