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10.1038/s41598-017-05018-4 http://scihub22266oqcxt.onion/10.1038/s41598-017-05018-4 C5496874!5496874 !28676704     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=28676704 &cmd=llinks): Failed to open stream: HTTP request failed! 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Biomaterial-Free Three-Dimensional Bioprinting of Cardiac Tissue using Human Induced Pluripotent Stem Cell Derived Cardiomyocytes |pdf=|usr=}}{{28676704 }} gab.com Text Biomaterial-Free Three-Dimensional Bioprinting of Cardiac Tissue using Human Induced Pluripotent Stem Cell Derived Cardiomyocytes JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=28676704 #FOAvip We have developed a novel method to deliver stem cells using 3D bioprinted cardiac patches, free of biomaterials. Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs), fibroblasts (FB) and endothelial cells (EC) were aggregated to create mixed cell spheroids. Cardiac patches were created from spheroids (CM:FB:EC?=?70:15:15, 70:0:30, 45:40:15) using a 3D bioprinter. Cardiac patches were analyzed with light and video microscopy, immunohistochemistry, immunofluorescence, cell viability assays and optical electrical mapping. Cardiac tissue patches of all cell ratios beat spontaneously after 3D bioprinting. Patches exhibited ventricular-like action potential waveforms and uniform electrical conduction throughout the patch. Conduction velocities were higher and action potential durations were significantly longer in patches containing a lower percentage of FBs. Immunohistochemistry revealed staining for CM, FB and EC markers, with rudimentary CD31+ blood vessel formation. Immunofluorescence revealed the presence of Cx43, the main cardiac gap junction protein, localized to cell-cell borders. In vivo implantation suggests vascularization of 3D bioprinted cardiac patches with engraftment into native rat myocardium. This constitutes a significant step towards a new generation of stem cell-based treatment for heart failure. Twit Text FOAVip Biomaterial-Free Three-Dimensional Bioprinting of Cardiac Tissue using Human Induced Pluripotent Stem Cell Derived Cardiomyocytes ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=28676704 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28676704 #FOAvip English Wikipedia <ref>{{Cite pmid|28676704 }}</ref> Biomaterial-Free Three-Dimensional Bioprinting of Cardiac Tissue using Human Induced Pluripotent Stem Cell Derived Cardiomyocytes #MMPMID28676704 Ong CS ; Fukunishi T ; Zhang H ; Huang CY ; Nashed A ; Blazeski A ; DiSilvestre D ; Vricella L ; Conte J ; Tung L ; Tomaselli GF ; Hibino N Sci Rep 2017[Jul]; 7 (1 ): 4566 PMID28676704 show ga We have developed a novel method to deliver stem cells using 3D bioprinted cardiac patches, free of biomaterials. Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs), fibroblasts (FB) and endothelial cells (EC) were aggregated to create mixed cell spheroids. Cardiac patches were created from spheroids (CM:FB:EC?=?70:15:15, 70:0:30, 45:40:15) using a 3D bioprinter. Cardiac patches were analyzed with light and video microscopy, immunohistochemistry, immunofluorescence, cell viability assays and optical electrical mapping. Cardiac tissue patches of all cell ratios beat spontaneously after 3D bioprinting. Patches exhibited ventricular-like action potential waveforms and uniform electrical conduction throughout the patch. Conduction velocities were higher and action potential durations were significantly longer in patches containing a lower percentage of FBs. Immunohistochemistry revealed staining for CM, FB and EC markers, with rudimentary CD31+ blood vessel formation. Immunofluorescence revealed the presence of Cx43, the main cardiac gap junction protein, localized to cell-cell borders. In vivo implantation suggests vascularization of 3D bioprinted cardiac patches with engraftment into native rat myocardium. This constitutes a significant step towards a new generation of stem cell-based treatment for heart failure. |*Biocompatible Materials/chemistry [MESH] |*Bioprinting [MESH] |*Induced Pluripotent Stem Cells/cytology [MESH] |*Myocytes, Cardiac/cytology/physiology [MESH] |*Printing, Three-Dimensional [MESH] |*Tissue Engineering [MESH] |*Tissue Scaffolds [MESH] |Animals [MESH] |Cell Differentiation [MESH] |Cells, Cultured [MESH] |Electrophysiological Phenomena [MESH] |Endothelial Cells [MESH] |Fibroblasts/cytology/metabolism [MESH] |Myocardium/cytology/metabolism [MESH] |Rats [MESH] |Spheroids, Cellular [MESH]Biomaterial-Free Three-Dimensional Bioprinting of Cardiac Tissue using(epmc).pdf DeepDyve Pubget Overpricing