How to speed up the polymerase chain reaction #MMPMID28702368
Bustin SA
Biomol Detect Quantif 2017[Jun]; 12 (ä): 10-4 PMID28702368show ga
Reducing the time taken to run qPCR assays on today?s qPCR cyclers is rather straightforward and requires no specialised reagents or instruments. As the first article in a new series of short technical reports, I demonstrate that it is possible to reduce significantly both denaturation temperatures and cycling times, whilst retaining sensitivity and specificity of the original qPCR conditions.