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Sophisticated Cloning, Fermentation, and Purification Technologies for an
Enhanced Therapeutic Protein Production: A Review
#MMPMID28725194
Gupta SK
; Shukla P
Front Pharmacol
2017[]; 8
(?): 419
PMID28725194
show ga
The protein productions strategies are crucial towards the development of
application based research and elucidating the novel purification strategies for
industrial production. Currently, there are few innovative avenues are studies
for cloning, upstream, and purification through efficient bioprocess development.
Such strategies are beneficial for industries as well as proven to be vital for
effectual therapeutic protein development. Though, these techniques are well
documented, but, there is scope of addition to current knowledge with novel and
new approaches and it will pave new avenues in production of recombinant
microbial and non-microbial proteins including secondary metabolites. In this
review, we have focussed on the recent development in clone selection, various
modern fermentation and purification technologies and future directions in these
emerging areas. Moreover, we have also highlighted notable perspectives and
challenges involved in the bioengineering of such proteins, including quality by
design, gene editing and pioneering ideas. The biopharmaceutical industries
continue to shift towards more flexible, automated platforms and economical
product development, which in turn can help in developing the cost effective
processes and affordable drug development for a large community.
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