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10.1167/iovs.16-20796 http://scihub22266oqcxt.onion/10.1167/iovs.16-20796 C5495152!5495152 !28672397     free     free     free Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\28672397 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Invest+Ophthalmol+Vis+Sci 2017 ; 58 (9 ): 3311-3318 Nephropedia Template TP {{tp|p=28672397 |t=2017. Structural and Functional Characterization of Human Stem-Cell-Derived Retinal Organoids by Live Imaging |pdf=|usr=}}{{28672397 }} gab.com Text Structural and Functional Characterization of Human Stem-Cell-Derived Retinal Organoids by Live Imaging JO: Invest Ophthalmol Vis Sci http://www.kidney.de/pget.php?&tw=1&pmid=28672397 #FOAvip PURPOSE: Human pluripotent stem cell (hPSC)-derived retinal organoids are a platform for investigating retinal development, pathophysiology, and cellular therapies. In contrast to histologic analysis in which multiple specimens fixed at different times are used to reconstruct developmental processes, repeated analysis of the same living organoids provides a more direct means to characterize changes. New live imaging modalities can provide insights into retinal organoid structure and metabolic function during in vitro growth. This study employed live tissue imaging to characterize retinal organoid development, including metabolic changes accompanying photoreceptor differentiation. METHODS: Live hPSC-derived retinal organoids at different developmental stages were examined for microanatomic organization and metabolic function by phase contrast microscopy, optical coherence tomography (OCT), fluorescence lifetime imaging microscopy (FLIM), and hyperspectral imaging (HSpec). Features were compared to those revealed by histologic staining, immunostaining, and microcomputed tomography (micro-CT) of fixed organoid tissue. RESULTS: We used FLIM and HSpec to detect changes in metabolic activity as organoids differentiated into organized lamellae. FLIM detected increased glycolytic activity and HSpec detected retinol and retinoic acid accumulation in the organoid outer layer, coinciding with photoreceptor genesis. OCT enabled imaging of lamellae formed during organoid maturation. Micro-CT revealed three-dimensional structure, but failed to detect lamellae. CONCLUSIONS: Live imaging modalities facilitate real-time and nondestructive imaging of retinal organoids as they organize into lamellar structures. FLIM and HSpec enable rapid detection of lamellar structure and photoreceptor metabolism. Live imaging techniques may aid in the continuous evaluation of retinal organoid development in diverse experimental and cell therapy settings. Twit Text FOAVip Structural and Functional Characterization of Human Stem-Cell-Derived Retinal Organoids by Live Imaging ????Invest Ophthalmol Vis Sci http://www.kidney.de/pget.php?&tw=1&pmid=28672397 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28672397 #FOAvip English Wikipedia <ref>{{Cite pmid|28672397 }}</ref> Structural and Functional Characterization of Human Stem-Cell-Derived Retinal Organoids by Live Imaging #MMPMID28672397 Browne AW ; Arnesano C ; Harutyunyan N ; Khuu T ; Martinez JC ; Pollack HA ; Koos DS ; Lee TC ; Fraser SE ; Moats RA ; Aparicio JG ; Cobrinik D Invest Ophthalmol Vis Sci 2017[Jul]; 58 (9 ): 3311-3318 PMID28672397 show ga PURPOSE: Human pluripotent stem cell (hPSC)-derived retinal organoids are a platform for investigating retinal development, pathophysiology, and cellular therapies. In contrast to histologic analysis in which multiple specimens fixed at different times are used to reconstruct developmental processes, repeated analysis of the same living organoids provides a more direct means to characterize changes. New live imaging modalities can provide insights into retinal organoid structure and metabolic function during in vitro growth. This study employed live tissue imaging to characterize retinal organoid development, including metabolic changes accompanying photoreceptor differentiation. METHODS: Live hPSC-derived retinal organoids at different developmental stages were examined for microanatomic organization and metabolic function by phase contrast microscopy, optical coherence tomography (OCT), fluorescence lifetime imaging microscopy (FLIM), and hyperspectral imaging (HSpec). Features were compared to those revealed by histologic staining, immunostaining, and microcomputed tomography (micro-CT) of fixed organoid tissue. RESULTS: We used FLIM and HSpec to detect changes in metabolic activity as organoids differentiated into organized lamellae. FLIM detected increased glycolytic activity and HSpec detected retinol and retinoic acid accumulation in the organoid outer layer, coinciding with photoreceptor genesis. OCT enabled imaging of lamellae formed during organoid maturation. Micro-CT revealed three-dimensional structure, but failed to detect lamellae. CONCLUSIONS: Live imaging modalities facilitate real-time and nondestructive imaging of retinal organoids as they organize into lamellar structures. FLIM and HSpec enable rapid detection of lamellar structure and photoreceptor metabolism. Live imaging techniques may aid in the continuous evaluation of retinal organoid development in diverse experimental and cell therapy settings. |*Diagnostic Techniques, Ophthalmological [MESH] |Humans [MESH] |Microscopy, Fluorescence/methods [MESH] |Organoids/*diagnostic imaging [MESH] |Pluripotent Stem Cells/*cytology [MESH] |Retina/*cytology/diagnostic imaging [MESH] |Tomography, Optical Coherence [MESH]Structural and Functional Characterization of Human Stem-Cell-Derived (epmc).pdf DeepDyve Pubget Overpricing