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10.15252/emmm.201707550 http://scihub22266oqcxt.onion/10.15252/emmm.201707550 C5494503!5494503!28468934     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       EMBO+Mol+Med 2017 ; 9 (7): 869-79 Nephropedia Template TP {{tp|p=28468934|t=2017. A genome editing approach to study cancer stem cells in human tumors |pdf=|usr=}}{{28468934}} gab.com Text A genome editing approach to study cancer stem cells in human tumors JO: EMBO Mol Med http://www.kidney.de/pget.php?&tw=1&pmid=28468934 #FOAvip The analysis of stem cell hierarchies in human cancers has been hampered by the impossibility of identifying or tracking tumor cell populations in an intact environment. To overcome this limitation, we devised a strategy based on editing the genomes of patient?derived tumor organoids using CRISPR/Cas9 technology to integrate reporter cassettes at desired marker genes. As proof of concept, we engineered human colorectal cancer (CRC) organoids that carry EGFP and lineage?tracing cassettes knocked in the LGR5 locus. Analysis of LGR5?EGFP+ cells isolated from organoid?derived xenografts demonstrated that these cells express a gene program similar to that of normal intestinal stem cells and that they propagate the disease to recipient mice very efficiently. Lineage?tracing experiments showed that LGR5+CRC cells self?renew and generate progeny over long time periods that undergo differentiation toward mucosecreting? and absorptive?like phenotypes. These genetic experiments confirm that human CRCs adopt a hierarchical organization reminiscent of that of the normal colonic epithelium. The strategy described herein may have broad applications to study cell heterogeneity in human tumors. Twit Text FOAVip A genome editing approach to study cancer stem cells in human tumors ????EMBO Mol Med http://www.kidney.de/pget.php?&tw=1&pmid=28468934 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28468934 #FOAvip English Wikipedia <ref>{{Cite pmid|28468934}}</ref> A genome editing approach to study cancer stem cells in human tumors #MMPMID28468934 Cortina C; Turon G; Stork D; Hernando?Momblona X; Sevillano M; Aguilera M; Tosi S; Merlos?Suárez A; Stephan?Otto Attolini C; Sancho E; Batlle E EMBO Mol Med 2017[Jul]; 9 (7): 869-79 PMID28468934show ga The analysis of stem cell hierarchies in human cancers has been hampered by the impossibility of identifying or tracking tumor cell populations in an intact environment. To overcome this limitation, we devised a strategy based on editing the genomes of patient?derived tumor organoids using CRISPR/Cas9 technology to integrate reporter cassettes at desired marker genes. As proof of concept, we engineered human colorectal cancer (CRC) organoids that carry EGFP and lineage?tracing cassettes knocked in the LGR5 locus. Analysis of LGR5?EGFP+ cells isolated from organoid?derived xenografts demonstrated that these cells express a gene program similar to that of normal intestinal stem cells and that they propagate the disease to recipient mice very efficiently. Lineage?tracing experiments showed that LGR5+CRC cells self?renew and generate progeny over long time periods that undergo differentiation toward mucosecreting? and absorptive?like phenotypes. These genetic experiments confirm that human CRCs adopt a hierarchical organization reminiscent of that of the normal colonic epithelium. The strategy described herein may have broad applications to study cell heterogeneity in human tumors. äA genome editing approach to study cancer stem cells in human tumors (epmc).pdf DeepDyve Pubget Overpricing