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10.1038/s41598-017-04066-0 http://scihub22266oqcxt.onion/10.1038/s41598-017-04066-0 C5493633!5493633!28667313     free     free     free Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Sci+Rep 2017 ; 7 (ä): ä Nephropedia Template TP {{tp|p=28667313|t=2017. Nanoparticles for live cell microscopy: A surface-enhanced Raman scattering perspective |pdf=|usr=}}{{28667313}} gab.com Text Nanoparticles for live cell microscopy: A surface-enhanced Raman scattering perspective JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=28667313 #FOAvip Surface enhanced Raman scattering (SERS) nanoparticles are an attractive alternative to fluorescent probes for biological labeling because of their photostability and multiplexing capabilities. However, nanoparticle size, shape, and surface properties are known to affect nanoparticle-cell interactions. Other issues such as the formation of a protein corona and antibody multivalency interfere with the labeling properties of nanoparticle-antibody conjugates. Hence, it is important to consider these aspects in order to validate such conjugates for live cell imaging applications. Using SERS nanoparticles that target HER2 and CD44 in breast cancer cells, we demonstrate labeling of fixed cells with high specificity that correlates well with fluorescent labels. However, when labeling live cells to monitor surface biomarker expression and dynamics, the nanoparticles are rapidly uptaken by the cells and become compartmentalized into different cellular regions. This behavior is in stark contrast to that of fluorescent antibody conjugates. This study highlights the impact of nanoparticle internalization and trafficking on the ability to use SERS nanoparticle-antibody conjugates to monitor cell dynamics. Twit Text FOAVip Nanoparticles for live cell microscopy: A surface-enhanced Raman scattering perspective ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=28667313 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28667313 #FOAvip English Wikipedia <ref>{{Cite pmid|28667313}}</ref> Nanoparticles for live cell microscopy: A surface-enhanced Raman scattering perspective #MMPMID28667313 Navas-Moreno M; Mehrpouyan M; Chernenko T; Candas D; Fan M; Li JJ; Yan M; Chan JW Sci Rep 2017[]; 7 (ä): ä PMID28667313show ga Surface enhanced Raman scattering (SERS) nanoparticles are an attractive alternative to fluorescent probes for biological labeling because of their photostability and multiplexing capabilities. However, nanoparticle size, shape, and surface properties are known to affect nanoparticle-cell interactions. Other issues such as the formation of a protein corona and antibody multivalency interfere with the labeling properties of nanoparticle-antibody conjugates. Hence, it is important to consider these aspects in order to validate such conjugates for live cell imaging applications. Using SERS nanoparticles that target HER2 and CD44 in breast cancer cells, we demonstrate labeling of fixed cells with high specificity that correlates well with fluorescent labels. However, when labeling live cells to monitor surface biomarker expression and dynamics, the nanoparticles are rapidly uptaken by the cells and become compartmentalized into different cellular regions. This behavior is in stark contrast to that of fluorescent antibody conjugates. This study highlights the impact of nanoparticle internalization and trafficking on the ability to use SERS nanoparticle-antibody conjugates to monitor cell dynamics. äNanoparticles for live cell microscopy: A surface-enhanced Raman scatt(epmc).pdf DeepDyve Pubget Overpricing