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10.1091/mbc.E16-12-0862 http://scihub22266oqcxt.onion/10.1091/mbc.E16-12-0862 C5491184!5491184 !28495796     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\28495796 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Mol+Biol+Cell 2017 ; 28 (13 ): 1754-1767 Nephropedia Template TP {{tp|p=28495796 |t=2017. Phosphorylation of NHE3-S(719) regulates NHE3 activity through the formation of multiple signaling complexes |pdf=|usr=}}{{28495796 }} gab.com Text Phosphorylation of NHE3-S(719) regulates NHE3 activity through the formation of multiple signaling complexes JO: Mol Biol Cell http://www.kidney.de/pget.php?&tw=1&pmid=28495796 #FOAvip Casein kinase 2 (CK2) binds to the NHE3 C-terminus and constitutively phosphorylates a downstream site (S719) that accounts for 40% of basal NHE3 activity. The role of CK2 in regulation of NHE3 activity in polarized Caco-2/bbe cells was further examined by mutation of NHE3-S(719) to A (not phosphorylated) or D (phosphomimetic). NHE3-S719A but not -S719D had multiple changes in NHE3 activity: 1) reduced basal NHE3 activity-specifically, inhibition of the PI3K/AKT-dependent component; 2) reduced acute stimulation of NHE3 activity by LPA/LPA(5)R stimulation; and 3) reduced acute inhibition of NHE3 activity-specifically, elevated Ca(2+) related (carbachol/Ca(2+) ionophore), but there was normal inhibition by forskolin and hyperosmolarity. The S719A mutant had reduced NHE3 complex size, reduced expression in lipid rafts, increased BB mobile fraction, and reduced binding to multiple proteins that bind throughout the NHE3 intracellular C-terminus, including calcineurin homologous protein, the NHERF family and SNX27 (related PDZ domains). These studies show that phosphorylation of the NHE3 at a single amino acid in the distal part of the C-terminus affects multiple aspects of NHE3 complex formation and changes the NHE3 lipid raft distribution, which cause changes in specific aspects of basal as well as acutely stimulated and inhibited Na(+)/H(+) exchange activity. Twit Text FOAVip Phosphorylation of NHE3-S(719) regulates NHE3 activity through the formation of multiple signaling complexes ????Mol Biol Cell http://www.kidney.de/pget.php?&tw=1&pmid=28495796 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28495796 #FOAvip English Wikipedia <ref>{{Cite pmid|28495796 }}</ref> Phosphorylation of NHE3-S(719) regulates NHE3 activity through the formation of multiple signaling complexes #MMPMID28495796 Sarker R ; Cha B ; Kovbasnjuk O ; Cole R ; Gabelli S ; Tse CM ; Donowitz M Mol Biol Cell 2017[Jul]; 28 (13 ): 1754-1767 PMID28495796 show ga Casein kinase 2 (CK2) binds to the NHE3 C-terminus and constitutively phosphorylates a downstream site (S719) that accounts for 40% of basal NHE3 activity. The role of CK2 in regulation of NHE3 activity in polarized Caco-2/bbe cells was further examined by mutation of NHE3-S(719) to A (not phosphorylated) or D (phosphomimetic). NHE3-S719A but not -S719D had multiple changes in NHE3 activity: 1) reduced basal NHE3 activity-specifically, inhibition of the PI3K/AKT-dependent component; 2) reduced acute stimulation of NHE3 activity by LPA/LPA(5)R stimulation; and 3) reduced acute inhibition of NHE3 activity-specifically, elevated Ca(2+) related (carbachol/Ca(2+) ionophore), but there was normal inhibition by forskolin and hyperosmolarity. The S719A mutant had reduced NHE3 complex size, reduced expression in lipid rafts, increased BB mobile fraction, and reduced binding to multiple proteins that bind throughout the NHE3 intracellular C-terminus, including calcineurin homologous protein, the NHERF family and SNX27 (related PDZ domains). These studies show that phosphorylation of the NHE3 at a single amino acid in the distal part of the C-terminus affects multiple aspects of NHE3 complex formation and changes the NHE3 lipid raft distribution, which cause changes in specific aspects of basal as well as acutely stimulated and inhibited Na(+)/H(+) exchange activity. |Caco-2 Cells [MESH] |Calcium/metabolism [MESH] |Carbachol/metabolism [MESH] |Casein Kinase II/*metabolism [MESH] |Epithelial Cells/metabolism [MESH] |Exocytosis [MESH] |Humans [MESH] |Lysophospholipids/metabolism [MESH] |Phosphatidylinositol 3-Kinases/metabolism [MESH] |Phosphoproteins [MESH] |Phosphorylation [MESH] |Protein Binding [MESH] |Protein Domains [MESH] |Protein Transport [MESH] |Proto-Oncogene Proteins c-akt/metabolism [MESH] |Signal Transduction [MESH] |Sodium-Hydrogen Exchanger 3/genetics/*metabolism [MESH] |Sodium-Hydrogen Exchangers [MESH]Phosphorylation of NHE3-S(719) regulates NHE3 activity through the for(epmc).pdf DeepDyve Pubget Overpricing