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10.1091/mbc.E16-06-0389 http://scihub22266oqcxt.onion/10.1091/mbc.E16-06-0389 C5491183!5491183 !28515142     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=28515142 &cmd=llinks): Failed to open stream: HTTP request failed! 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Flow-induced endothelial cell alignment requires the RhoGEF Trio as a scaffold protein to polarize active Rac1 distribution |pdf=|usr=}}{{28515142 }} gab.com Text Flow-induced endothelial cell alignment requires the RhoGEF Trio as a scaffold protein to polarize active Rac1 distribution JO: Mol Biol Cell http://www.kidney.de/pget.php?&tw=1&pmid=28515142 #FOAvip Endothelial cells line the lumen of the vessel wall and are exposed to flow. In linear parts of the vessel, the endothelial cells experience laminar flow, resulting in endothelial cell alignment in the direction of flow, thereby protecting the vessel wall from inflammation and permeability. In order for endothelial cells to align, they undergo rapid remodeling of the actin cytoskeleton by local activation of the small GTPase Rac1. However, it is not clear whether sustained and local activation of Rac1 is required for long-term flow-induced cell alignment. Using a FRET-based DORA Rac1 biosensor, we show that local Rac1 activity remains for 12 h upon long-term flow. Silencing studies show that the RhoGEF Trio is crucial for keeping active Rac1 at the downstream side of the cell and, as a result, for long-term flow-induced cell alignment. Surprisingly, Trio appears to be not involved in flow-induced activation of Rac1. Our data show that flow induces Rac1 activity at the downstream side of the cell in a Trio-dependent manner and that Trio functions as a scaffold protein rather than a functional GEF under long-term flow conditions. Twit Text FOAVip Flow-induced endothelial cell alignment requires the RhoGEF Trio as a scaffold protein to polarize active Rac1 distribution ????Mol Biol Cell http://www.kidney.de/pget.php?&tw=1&pmid=28515142 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28515142 #FOAvip English Wikipedia <ref>{{Cite pmid|28515142 }}</ref> Flow-induced endothelial cell alignment requires the RhoGEF Trio as a scaffold protein to polarize active Rac1 distribution #MMPMID28515142 Kroon J ; Heemskerk N ; Kalsbeek MJT ; de Waard V ; van Rijssel J ; van Buul JD Mol Biol Cell 2017[Jul]; 28 (13 ): 1745-1753 PMID28515142 show ga Endothelial cells line the lumen of the vessel wall and are exposed to flow. In linear parts of the vessel, the endothelial cells experience laminar flow, resulting in endothelial cell alignment in the direction of flow, thereby protecting the vessel wall from inflammation and permeability. In order for endothelial cells to align, they undergo rapid remodeling of the actin cytoskeleton by local activation of the small GTPase Rac1. However, it is not clear whether sustained and local activation of Rac1 is required for long-term flow-induced cell alignment. Using a FRET-based DORA Rac1 biosensor, we show that local Rac1 activity remains for 12 h upon long-term flow. Silencing studies show that the RhoGEF Trio is crucial for keeping active Rac1 at the downstream side of the cell and, as a result, for long-term flow-induced cell alignment. Surprisingly, Trio appears to be not involved in flow-induced activation of Rac1. Our data show that flow induces Rac1 activity at the downstream side of the cell in a Trio-dependent manner and that Trio functions as a scaffold protein rather than a functional GEF under long-term flow conditions. |Actin Cytoskeleton/metabolism [MESH] |Actins/metabolism [MESH] |Cells, Cultured [MESH] |Endothelial Cells/metabolism [MESH] |Human Umbilical Vein Endothelial Cells [MESH] |Humans [MESH] |Hydrodynamics [MESH] |Phosphoproteins/metabolism [MESH] |Protein Serine-Threonine Kinases/metabolism [MESH] |Rho Guanine Nucleotide Exchange Factors/*metabolism [MESH] |Signal Transduction [MESH]Flow-induced endothelial cell alignment requires the RhoGEF Trio as a (epmc).pdf DeepDyve Pubget Overpricing