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10.1186/s13059-017-1247-6 http://scihub22266oqcxt.onion/10.1186/s13059-017-1247-6 C5488340!5488340!28655330     free     free     free       Genome+Biol 2017 ; 18 (ä): ä Nephropedia Template TP {{tp|p=28655330|t=2017. NicE-seq: high resolution open chromatin profiling |pdf=|usr=}}{{28655330}} gab.com Text NicE-seq: high resolution open chromatin profiling JO: Genome Biol http://www.kidney.de/pget.php?&tw=1&pmid=28655330 #FOAvip Open chromatin profiling integrates information across diverse regulatory elements to reveal the transcriptionally active genome. Tn5 transposase and DNase I sequencing-based methods prefer native or high cell numbers. Here, we describe NicE-seq (nicking enzyme assisted sequencing) for high-resolution open chromatin profiling on both native and formaldehyde-fixed cells. NicE-seq captures and reveals open chromatin sites (OCSs) and transcription factor occupancy at single nucleotide resolution, coincident with DNase hypersensitive and ATAC-seq sites at a low sequencing burden. OCSs correlate with RNA polymerase II occupancy and active chromatin marks, while displaying a contrasting pattern to CpG methylation. Decitabine-mediated hypomethylation of HCT116 displays higher numbers of OCSs.Electronic supplementary material: The online version of this article (doi:10.1186/s13059-017-1247-6) contains supplementary material, which is available to authorized users. Twit Text FOAVip NicE-seq: high resolution open chromatin profiling ????Genome Biol http://www.kidney.de/pget.php?&tw=1&pmid=28655330 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28655330 #FOAvip English Wikipedia <ref>{{Cite pmid|28655330}}</ref> NicE-seq: high resolution open chromatin profiling #MMPMID28655330 Ponnaluri VKC; Zhang G; Estève PO; Spracklin G; Sian S; Xu Sy; Benoukraf T; Pradhan S Genome Biol 2017[]; 18 (ä): ä PMID28655330show ga Open chromatin profiling integrates information across diverse regulatory elements to reveal the transcriptionally active genome. Tn5 transposase and DNase I sequencing-based methods prefer native or high cell numbers. Here, we describe NicE-seq (nicking enzyme assisted sequencing) for high-resolution open chromatin profiling on both native and formaldehyde-fixed cells. NicE-seq captures and reveals open chromatin sites (OCSs) and transcription factor occupancy at single nucleotide resolution, coincident with DNase hypersensitive and ATAC-seq sites at a low sequencing burden. OCSs correlate with RNA polymerase II occupancy and active chromatin marks, while displaying a contrasting pattern to CpG methylation. Decitabine-mediated hypomethylation of HCT116 displays higher numbers of OCSs.Electronic supplementary material: The online version of this article (doi:10.1186/s13059-017-1247-6) contains supplementary material, which is available to authorized users. äNicE-seq: high resolution open chromatin profiling (epmc).pdf DeepDyve Pubget Overpricing