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10.1128/JVI.79.5.2847-2858.2005 http://scihub22266oqcxt.onion/10.1128/JVI.79.5.2847-2858.2005 C548444!548444 !15709004     free     free     free Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 263.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 263.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\15709004 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       J+Virol 2005 ; 79 (5 ): 2847-58 Nephropedia Template TP {{tp|p=15709004 |t=2005. Involvement of the Toll-like receptor 9 signaling pathway in the induction of innate immunity by baculovirus |pdf=|usr=}}{{15709004 }} gab.com Text Involvement of the Toll-like receptor 9 signaling pathway in the induction of innate immunity by baculovirus JO: J Virol http://www.kidney.de/pget.php?&tw=1&pmid=15709004 #FOAvip We have previously shown that mice inoculated intranasally with a wild-type baculovirus (Autographa californica nuclear polyhedrosis virus [AcNPV]) are protected from a lethal challenge by influenza virus. However, the precise mechanism of induction of this protective immune response by the AcNPV treatment remained unclear. Here we show that AcNPV activates immune cells via the Toll-like receptor 9 (TLR9)/MyD88-dependent signaling pathway. The production of inflammatory cytokines was severely reduced in peritoneal macrophages (PECs) and splenic CD11c(+) dendritic cells (DCs) derived from mice deficient in MyD88 or TLR9 after cultivation with AcNPV. In contrast, a significant amount of alpha interferon (IFN-alpha) was still detectable in the PECs and DCs of these mice after stimulation with AcNPV, suggesting that a TLR9/MyD88-independent signaling pathway might also participate in the production of IFN-alpha by AcNPV. Since previous work showed that TLR9 ligands include bacterial DNA and certain oligonucleotides containing unmethylated CpG dinucleotides, we also examined the effect of baculoviral DNA on the induction of innate immunity. Transfection of the murine macrophage cell line RAW264.7 with baculoviral DNA resulted in the production of the inflammatory cytokine, while the removal of envelope glycoproteins from viral particles, UV irradiation of the virus, and pretreatment with purified baculovirus envelope proteins or endosomal maturation inhibitors diminished the induction of the immune response by AcNPV. Together, these results indicate that the internalization of viral DNA via membrane fusion mediated by the viral envelope glycoprotein, as well as endosomal maturation, which releases the viral genome into TLR9-expressing cellular compartments, is necessary for the induction of the innate immune response by AcNPV. Twit Text FOAVip Involvement of the Toll-like receptor 9 signaling pathway in the induction of innate immunity by baculovirus ????J Virol http://www.kidney.de/pget.php?&tw=1&pmid=15709004 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=15709004 #FOAvip English Wikipedia <ref>{{Cite pmid|15709004 }}</ref> Involvement of the Toll-like receptor 9 signaling pathway in the induction of innate immunity by baculovirus #MMPMID15709004 Abe T ; Hemmi H ; Miyamoto H ; Moriishi K ; Tamura S ; Takaku H ; Akira S ; Matsuura Y J Virol 2005[Mar]; 79 (5 ): 2847-58 PMID15709004 show ga We have previously shown that mice inoculated intranasally with a wild-type baculovirus (Autographa californica nuclear polyhedrosis virus [AcNPV]) are protected from a lethal challenge by influenza virus. However, the precise mechanism of induction of this protective immune response by the AcNPV treatment remained unclear. Here we show that AcNPV activates immune cells via the Toll-like receptor 9 (TLR9)/MyD88-dependent signaling pathway. The production of inflammatory cytokines was severely reduced in peritoneal macrophages (PECs) and splenic CD11c(+) dendritic cells (DCs) derived from mice deficient in MyD88 or TLR9 after cultivation with AcNPV. In contrast, a significant amount of alpha interferon (IFN-alpha) was still detectable in the PECs and DCs of these mice after stimulation with AcNPV, suggesting that a TLR9/MyD88-independent signaling pathway might also participate in the production of IFN-alpha by AcNPV. Since previous work showed that TLR9 ligands include bacterial DNA and certain oligonucleotides containing unmethylated CpG dinucleotides, we also examined the effect of baculoviral DNA on the induction of innate immunity. Transfection of the murine macrophage cell line RAW264.7 with baculoviral DNA resulted in the production of the inflammatory cytokine, while the removal of envelope glycoproteins from viral particles, UV irradiation of the virus, and pretreatment with purified baculovirus envelope proteins or endosomal maturation inhibitors diminished the induction of the immune response by AcNPV. Together, these results indicate that the internalization of viral DNA via membrane fusion mediated by the viral envelope glycoprotein, as well as endosomal maturation, which releases the viral genome into TLR9-expressing cellular compartments, is necessary for the induction of the innate immune response by AcNPV. |*Immunity, Innate [MESH] |Adaptor Proteins, Signal Transducing [MESH] |Animals [MESH] |Antigens, Differentiation/genetics/physiology [MESH] |Base Sequence [MESH] |Cell Line [MESH] |Cytokines/biosynthesis [MESH] |DNA, Viral/genetics [MESH] |DNA-Binding Proteins/deficiency/genetics/*physiology [MESH] |Dendritic Cells/immunology [MESH] |Endocytosis [MESH] |Humans [MESH] |Inflammation Mediators/metabolism [MESH] |Interferon-alpha/biosynthesis [MESH] |Macrophage Activation [MESH] |Macrophages, Peritoneal/immunology [MESH] |Mice [MESH] |Mice, Inbred C57BL [MESH] |Mice, Knockout [MESH] |Myeloid Differentiation Factor 88 [MESH] |NF-kappa B/metabolism [MESH] |Nucleopolyhedroviruses/genetics/*immunology [MESH] |Phagocytosis [MESH] |Receptors, Cell Surface/deficiency/genetics/*physiology [MESH] |Receptors, Immunologic/deficiency/genetics/physiology [MESH] |Signal Transduction [MESH] |Toll-Like Receptor 9 [MESH] |Transfection [MESH]Involvement of the Toll-like receptor 9 signaling pathway in the induc(epmc).pdf DeepDyve Pubget Overpricing