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2017 ; 8
(22
): 36012-36019
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gab.com Text
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Renal mass biopsy using Raman spectroscopy identifies malignant and benign renal
tumors: potential for pre-operative diagnosis
#MMPMID28415596
Liu Y
; Du Z
; Zhang J
; Jiang H
Oncotarget
2017[May]; 8
(22
): 36012-36019
PMID28415596
show ga
The accuracy of renal mass biopsy to diagnose malignancy can be affected by
multiple factors. Here, we investigated the feasibility of Raman spectroscopy to
distinguish malignant and benign renal tumors using biopsy specimens. Samples
were collected from 63 patients who received radical or partial nephrectomy, mass
suspicious of cancer and distal parenchyma were obtained from resected kidney
using an 18-gauge biopsy needle. Four Raman spectra were obtained for each
sample, and Discriminant Analysis was applied for data analysis. A total of 383
Raman spectra were eventually gathered and each type of tumor had its
characteristic spectrum. Raman could separate tumoral and normal tissues with an
accuracy of 82.53%, and distinguish malignant and benign tumors with a
sensitivity of 91.79% and specificity of 71.15%. It could classify low-grade and
high-grade tumors with an accuracy of 86.98%. Besides, clear cell renal carcinoma
was differentiated with oncocytoma and angiomyolipoma with accuracy of 100% and
89.25%, respectively. And histological subtypes of cell carcinoma were
distinguished with an accuracy of 93.48%. When compared with final pathology and
biopsy, Raman spectroscopy was able to correctly identify 7 of 11 "missed" biopsy
diagnoses. These results suggested that Raman may serve as a promising
non-invasive approach in the future for pre-operative diagnosis.