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10.12688/f1000research.11581.1 http://scihub22266oqcxt.onion/10.12688/f1000research.11581.1 C5482341!5482341!28690838     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       F1000Res 2017 ; 6 (ä): ä Nephropedia Template TP {{tp|p=28690838|t=2017. Recent advances in high-throughput approaches to dissect enhancer function |pdf=|usr=}}{{28690838}} gab.com Text Recent advances in high-throughput approaches to dissect enhancer function JO: F1000Res http://www.kidney.de/pget.php?&tw=1&pmid=28690838 #FOAvip The regulation of gene transcription in higher eukaryotes is accomplished through the involvement of transcription start site (TSS)-proximal (promoters) and -distal (enhancers) regulatory elements. It is now well acknowledged that enhancer elements play an essential role during development and cell differentiation, while genetic alterations in these elements are a major cause of human disease. Many strategies have been developed to identify and characterize enhancers. Here, we discuss recent advances in high-throughput approaches to assess enhancer activity, from the well-established massively parallel reporter assays to the recent clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9-based technologies. We highlight how these approaches contribute toward a better understanding of enhancer function, eventually leading to the discovery of new types of regulatory sequences, and how the alteration of enhancers can affect transcriptional regulation. Twit Text FOAVip Recent advances in high-throughput approaches to dissect enhancer function ????F1000Res http://www.kidney.de/pget.php?&tw=1&pmid=28690838 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28690838 #FOAvip English Wikipedia <ref>{{Cite pmid|28690838}}</ref> Recent advances in high-throughput approaches to dissect enhancer function #MMPMID28690838 Santiago-Algarra D; Dao LT; Pradel L; Espaņa A; Spicuglia S F1000Res 2017[]; 6 (ä): ä PMID28690838show ga The regulation of gene transcription in higher eukaryotes is accomplished through the involvement of transcription start site (TSS)-proximal (promoters) and -distal (enhancers) regulatory elements. It is now well acknowledged that enhancer elements play an essential role during development and cell differentiation, while genetic alterations in these elements are a major cause of human disease. Many strategies have been developed to identify and characterize enhancers. Here, we discuss recent advances in high-throughput approaches to assess enhancer activity, from the well-established massively parallel reporter assays to the recent clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9-based technologies. We highlight how these approaches contribute toward a better understanding of enhancer function, eventually leading to the discovery of new types of regulatory sequences, and how the alteration of enhancers can affect transcriptional regulation. äRecent advances in high-throughput approaches to dissect enhancer func(epmc).pdf DeepDyve Pubget Overpricing