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10.4155/fsoa-2016-0088

http://scihub22266oqcxt.onion/10.4155/fsoa-2016-0088
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C5481810!5481810!28670469
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suck abstract from ncbi


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pmid28670469      Future+Sci+OA 2017 ; 3 (2): ä
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  • An integrated versatile lab-on-a-chip platform for the isolation and nucleic acid-based detection of pathogens #MMPMID28670469
  • Sandetskaya N; Moos D; Pötter H; Seifert S; Jenerowicz M; Becker H; Zilch C; Kuhlmeier D
  • Future Sci OA 2017[Jun]; 3 (2): ä PMID28670469show ga
  • Aim:: Processing of the samples in molecular diagnostics is complex and labor-intensive. An integrated and automated platform for sample preparation and nucleic acid-based detection can significantly relieve this burden for the users. Results:: We present a prototype of a versatile and integrated platform for the detection of pathogens in various liquid media. We describe a proof-of-concept for the integrated isolation of bacteria, cell lysis with optional DNA extraction, DNA amplification and detection in two different reactions, loop-mediated isothermal amplification and PCR, on a single microfluidic platform. Conclusion:: The platform enables the transition from large sample volume to microfluidic format. The design and open interface enable its versatile application for various nucleic acid-based assays, from simple to complex setups.
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