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2017 ; 3
(2
): FSO177
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An integrated versatile lab-on-a-chip platform for the isolation and nucleic
acid-based detection of pathogens
#MMPMID28670469
Sandetskaya N
; Moos D
; Pötter H
; Seifert S
; Jenerowicz M
; Becker H
; Zilch C
; Kuhlmeier D
Future Sci OA
2017[Jun]; 3
(2
): FSO177
PMID28670469
show ga
AIM: Processing of the samples in molecular diagnostics is complex and
labor-intensive. An integrated and automated platform for sample preparation and
nucleic acid-based detection can significantly relieve this burden for the users.
RESULTS: We present a prototype of a versatile and integrated platform for the
detection of pathogens in various liquid media. We describe a proof-of-concept
for the integrated isolation of bacteria, cell lysis with optional DNA
extraction, DNA amplification and detection in two different reactions,
loop-mediated isothermal amplification and PCR, on a single microfluidic
platform. CONCLUSION: The platform enables the transition from large sample
volume to microfluidic format. The design and open interface enable its versatile
application for various nucleic acid-based assays, from simple to complex setups.