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10.7554/eLife.15537 http://scihub22266oqcxt.onion/10.7554/eLife.15537 C5481204!5481204!27223327     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       eLife 2016 ; 5 (ä): ä Nephropedia Template TP {{tp|p=27223327|t=2016. A bend, flip and trap mechanism for transposon integration |pdf=|usr=}}{{27223327}} gab.com Text A bend, flip and trap mechanism for transposon integration JO: eLife http://www.kidney.de/pget.php?&tw=1&pmid=27223327 #FOAvip Cut-and-paste DNA transposons of the mariner/Tc1 family are useful tools for genome engineering and are inserted specifically at TA target sites. A crystal structure of the mariner transposase Mos1 (derived from Drosophila mauritiana), in complex with transposon ends covalently joined to target DNA, portrays the transposition machinery after DNA integration. It reveals severe distortion of target DNA and flipping of the target adenines into extra-helical positions. Fluorescence experiments confirm dynamic base flipping in solution. Transposase residues W159, R186, F187 and K190 stabilise the target DNA distortions and are required for efficient transposon integration and transposition in vitro. Transposase recognises the flipped target adenines via base-specific interactions with backbone atoms, offering a molecular basis for TA target sequence selection. Our results will provide a template for re-designing mariner/Tc1 transposases with modified target specificities.DOI:http://dx.doi.org/10.7554/eLife.15537.001 Twit Text FOAVip A bend, flip and trap mechanism for transposon integration ????eLife http://www.kidney.de/pget.php?&tw=1&pmid=27223327 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27223327 #FOAvip English Wikipedia <ref>{{Cite pmid|27223327}}</ref> A bend, flip and trap mechanism for transposon integration #MMPMID27223327 Morris ER; Grey H; McKenzie G; Jones AC; Richardson JM eLife 2016[]; 5 (ä): ä PMID27223327show ga Cut-and-paste DNA transposons of the mariner/Tc1 family are useful tools for genome engineering and are inserted specifically at TA target sites. A crystal structure of the mariner transposase Mos1 (derived from Drosophila mauritiana), in complex with transposon ends covalently joined to target DNA, portrays the transposition machinery after DNA integration. It reveals severe distortion of target DNA and flipping of the target adenines into extra-helical positions. Fluorescence experiments confirm dynamic base flipping in solution. Transposase residues W159, R186, F187 and K190 stabilise the target DNA distortions and are required for efficient transposon integration and transposition in vitro. Transposase recognises the flipped target adenines via base-specific interactions with backbone atoms, offering a molecular basis for TA target sequence selection. Our results will provide a template for re-designing mariner/Tc1 transposases with modified target specificities.DOI:http://dx.doi.org/10.7554/eLife.15537.001 äA bend, flip and trap mechanism for transposon integration (epmc).pdf DeepDyve Pubget Overpricing