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2016 ; 7
(ä): 13748
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Scanning superlens microscopy for non-invasive large field-of-view visible light
nanoscale imaging
#MMPMID27934860
Wang F
; Liu L
; Yu H
; Wen Y
; Yu P
; Liu Z
; Wang Y
; Li WJ
Nat Commun
2016[Dec]; 7
(ä): 13748
PMID27934860
show ga
Nanoscale correlation of structural information acquisition with
specific-molecule identification provides new insight for studying rare
subcellular events. To achieve this correlation, scanning electron microscopy has
been combined with super-resolution fluorescent microscopy, despite its
destructivity when acquiring biological structure information. Here we propose
time-efficient non-invasive microsphere-based scanning superlens microscopy that
enables the large-area observation of live-cell morphology or sub-membrane
structures with sub-diffraction-limited resolution and is demonstrated by
observing biological and non-biological objects. This microscopy operates in both
non-invasive and contact modes with ?200 times the acquisition efficiency of
atomic force microscopy, which is achieved by replacing the point of an atomic
force microscope tip with an imaging area of microspheres and stitching the areas
recorded during scanning, enabling sub-diffraction-limited resolution. Our method
marks a possible path to non-invasive cell imaging and simultaneous tracking of
specific molecules with nanoscale resolution, facilitating the study of
subcellular events over a total cell period.