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10.1002/pmic.201500526 http://scihub22266oqcxt.onion/10.1002/pmic.201500526 C5476226!5476226!27246681     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Proteomics 2016 ; 16 (15-16): 2257-71 Nephropedia Template TP {{tp|p=27246681|t=2016. Untargeted, spectral library-free analysis of data independent acquisition proteomics data generated using Orbitrap mass spectrometers |pdf=|usr=}}{{27246681}} gab.com Text Untargeted, spectral library-free analysis of data independent acquisition proteomics data generated using Orbitrap mass spectrometers JO: Proteomics http://www.kidney.de/pget.php?&tw=1&pmid=27246681 #FOAvip We describe an improved version of the data independent acquisition (DIA) computational analysis tool DIA-Umpire, and show that it enables highly sensitive, untargeted and direct (spectral library-free) analysis of DIA data obtained using the Orbitrap family of mass spectrometers. DIA-Umpire v2 implements an improved feature detection algorithm with two additional filters based on the isotope pattern and fractional peptide mass analysis. The targeted re-extraction step of DIA-Umpire is updated with an improved scoring function and a more robust, semi-parametric mixture modeling of the resulting scores for computing posterior probabilities of correct peptide identification in a targeted setting. Using two publicly available Q Exactive DIA datasets generated using HEK-293 cells and human liver microtissues, we demonstrate that DIA-Umpire can identify similar number of peptide ions, but with better identification reproducibility between replicates and samples, as with conventional data dependent acquisition (DDA). We further demonstrate the utility of DIA-Umpire using a series of Orbitrap Fusion DIA experiments with HeLa cell lysates profiled using conventional DDA and using DIA with different isolation window widths. Twit Text FOAVip Untargeted, spectral library-free analysis of data independent acquisition proteomics data generated using Orbitrap mass spectrometers ????Proteomics http://www.kidney.de/pget.php?&tw=1&pmid=27246681 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27246681 #FOAvip English Wikipedia <ref>{{Cite pmid|27246681}}</ref> Untargeted, spectral library-free analysis of data independent acquisition proteomics data generated using Orbitrap mass spectrometers #MMPMID27246681 Tsou CC; Tsai CF; Teo G; Chen YJ; Nesvizhskii AI Proteomics 2016[Aug]; 16 (15-16): 2257-71 PMID27246681show ga We describe an improved version of the data independent acquisition (DIA) computational analysis tool DIA-Umpire, and show that it enables highly sensitive, untargeted and direct (spectral library-free) analysis of DIA data obtained using the Orbitrap family of mass spectrometers. DIA-Umpire v2 implements an improved feature detection algorithm with two additional filters based on the isotope pattern and fractional peptide mass analysis. The targeted re-extraction step of DIA-Umpire is updated with an improved scoring function and a more robust, semi-parametric mixture modeling of the resulting scores for computing posterior probabilities of correct peptide identification in a targeted setting. Using two publicly available Q Exactive DIA datasets generated using HEK-293 cells and human liver microtissues, we demonstrate that DIA-Umpire can identify similar number of peptide ions, but with better identification reproducibility between replicates and samples, as with conventional data dependent acquisition (DDA). We further demonstrate the utility of DIA-Umpire using a series of Orbitrap Fusion DIA experiments with HeLa cell lysates profiled using conventional DDA and using DIA with different isolation window widths. äUntargeted, spectral library-free analysis of data independent acquisi(epmc).pdf DeepDyve Pubget Overpricing