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.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117 J+Phys+Chem+Lett
2013 ; 4
(17
): 2858-2866
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A Bright Light to Reveal Mobility: Single Quantum Dot Tracking Reveals Membrane
Dynamics and Cellular Mechanisms
#MMPMID28626534
Chang JC
; Rosenthal SJ
J Phys Chem Lett
2013[Aug]; 4
(17
): 2858-2866
PMID28626534
show ga
This perspective describes recent progress in single quantum dot techniques, with
an emphasis on their applications in exploring membrane dynamics and cellular
mechanisms. In these cases, conventional population measurements, such as
fluorescence recovery after photobleaching, yield only a mean value on an
ensemble or bulk collection of molecules, where the behavior of individual
proteins and vehicles is missing. In recent years, the single quantum dot imaging
approach has been introduced as a sub-category of single molecule fluorescent
techniques to reveal single protein/vehicle dynamics in real-time. One of the
major advantages of using single quantum dots is the high signal-to-noise ratio
originating from their unique photophysical properties such as extraordinarily
high molar extinction coefficients and large effective Stokes shifts. In addition
to a brief overview on the principle of single quantum dot imaging techniques, we
highlight recent discoveries and discuss future directions in the field.