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10.1038/ncomms15822 http://scihub22266oqcxt.onion/10.1038/ncomms15822 C5472792!5472792!28604727     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Commun 2017 ; 8 (ä): ä Nephropedia Template TP {{tp|p=28604727|t=2017. Bub1 positions Mad1 close to KNL1 MELT repeats to promote checkpoint signalling |pdf=|usr=}}{{28604727}} gab.com Text Bub1 positions Mad1 close to KNL1 MELT repeats to promote checkpoint signalling JO: Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=28604727 #FOAvip Proper segregation of chromosomes depends on a functional spindle assembly checkpoint (SAC) and requires kinetochore localization of the Bub1 and Mad1/Mad2 checkpoint proteins. Several aspects of Mad1/Mad2 kinetochore recruitment in human cells are unclear and in particular the underlying direct interactions. Here we show that conserved domain 1 (CD1) in human Bub1 binds directly to Mad1 and a phosphorylation site exists in CD1 that stimulates Mad1 binding and SAC signalling. Importantly, fusion of minimal kinetochore-targeting Bub1 fragments to Mad1 bypasses the need for CD1, revealing that the main function of Bub1 is to position Mad1 close to KNL1 MELT repeats. Furthermore, we identify residues in Mad1 that are critical for Mad1 functionality, but not Bub1 binding, arguing for a direct role of Mad1 in the checkpoint. This work dissects functionally relevant molecular interactions required for spindle assembly checkpoint signalling at kinetochores in human cells. Twit Text FOAVip Bub1 positions Mad1 close to KNL1 MELT repeats to promote checkpoint signalling ????Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=28604727 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28604727 #FOAvip English Wikipedia <ref>{{Cite pmid|28604727}}</ref> Bub1 positions Mad1 close to KNL1 MELT repeats to promote checkpoint signalling #MMPMID28604727 Zhang G; Kruse T; López-Méndez B; Sylvestersen KB; Garvanska DH; Schopper S; Nielsen ML; Nilsson J Nat Commun 2017[]; 8 (ä): ä PMID28604727show ga Proper segregation of chromosomes depends on a functional spindle assembly checkpoint (SAC) and requires kinetochore localization of the Bub1 and Mad1/Mad2 checkpoint proteins. Several aspects of Mad1/Mad2 kinetochore recruitment in human cells are unclear and in particular the underlying direct interactions. Here we show that conserved domain 1 (CD1) in human Bub1 binds directly to Mad1 and a phosphorylation site exists in CD1 that stimulates Mad1 binding and SAC signalling. Importantly, fusion of minimal kinetochore-targeting Bub1 fragments to Mad1 bypasses the need for CD1, revealing that the main function of Bub1 is to position Mad1 close to KNL1 MELT repeats. Furthermore, we identify residues in Mad1 that are critical for Mad1 functionality, but not Bub1 binding, arguing for a direct role of Mad1 in the checkpoint. This work dissects functionally relevant molecular interactions required for spindle assembly checkpoint signalling at kinetochores in human cells. äBub1 positions Mad1 close to KNL1 MELT repeats to promote checkpoint s(epmc).pdf DeepDyve Pubget Overpricing