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10.1186/s13075-017-1343-8 http://scihub22266oqcxt.onion/10.1186/s13075-017-1343-8 C5471953!5471953 !28615072     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=28615072 &cmd=llinks): Failed to open stream: HTTP request failed! 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Chemokine receptor co-expression reveals aberrantly distributed T(H) effector memory cells in GPA patients |pdf=|usr=}}{{28615072 }} gab.com Text Chemokine receptor co-expression reveals aberrantly distributed T(H) effector memory cells in GPA patients JO: Arthritis Res Ther http://www.kidney.de/pget.php?&tw=1&pmid=28615072 #FOAvip BACKGROUND: Persistent expansion of circulating CD4(+) effector memory T cells (T(EM)) in patients with granulomatosis with polyangiitis (GPA) suggests their fundamental role in disease pathogenesis. Recent studies have shown that distinct functional CD4(+) T(EM) cell subsets can be identified based on expression patterns of chemokine receptors. The current study aimed to determine different CD4(+) T(EM) cell subsets based on chemokine receptor expression in peripheral blood of GPA patients. Identification of particular circulating CD4(+) T(EM) cells subsets may reveal distinct contributions of specific CD4(+) T(EM) subsets to the disease pathogenesis in GPA. METHOD: Peripheral blood of 63 GPA patients in remission and 42 age- and sex-matched healthy controls was stained immediately after blood withdrawal with fluorochrome-conjugated antibodies for cell surface markers (CD3, CD4, CD45RO) and chemokine receptors (CCR4, CCR6, CCR7, CRTh2, CXCR3) followed by flow cytometry analysis. CD4(+) T(EM) memory cells (CD3(+)CD4(+)CD45RO(+)CCR7(-)) were gated, and the expression patterns of chemokine receptors CXCR3(+)CCR4(-)CCR6(-)CRTh2(-), CXCR3(-)CCR4(+)CCR6(-)CRTh2(+), CXCR3(-)CCR4(+)CCR6(+)CRTh2(-), and CXCR3(+)CCR4(-)CCR6(+)CRTh2(-) were used to distinguish T(EM)1, T(EM)2, T(EM)17, and T(EM)17.1 cells, respectively. RESULTS: The percentage of CD4(+) T(EM) cells was significantly increased in GPA patients in remission compared to HCs. Chemokine receptor co-expression analysis within the CD4(+) T(EM) cell population demonstrated a significant increase in the proportion of T(EM)17 cells with a concomitant significant decrease in the T(EM)1 cells in GPA patients compared to HC. The percentage of T(EM)17 cells correlated negatively with T(EM)1 cells in GPA patients. Moreover, the circulating proportion of T(EM)17 cells showed a positive correlation with the number of organs involved and an association with the tendency to relapse in GPA patients. Interestingly, the aberrant distribution of T(EM)1 and T(EM)17 cells is modulated in CMV- seropositive GPA patients. CONCLUSIONS: Our data demonstrates the identification of different CD4(+) T(EM) cell subsets in peripheral blood of GPA patients based on chemokine receptor co-expression analysis. The aberrant balance between T(EM)1 and T(EM)17 cells in remission GPA patients, showed to be associated with disease pathogenesis in relation to organ involvement, and tendency to relapse. Twit Text FOAVip Chemokine receptor co-expression reveals aberrantly distributed T(H) effector memory cells in GPA patients ????Arthritis Res Ther http://www.kidney.de/pget.php?&tw=1&pmid=28615072 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28615072 #FOAvip English Wikipedia <ref>{{Cite pmid|28615072 }}</ref> Chemokine receptor co-expression reveals aberrantly distributed T(H) effector memory cells in GPA patients #MMPMID28615072 Lintermans LL ; Rutgers A ; Stegeman CA ; Heeringa P ; Abdulahad WH Arthritis Res Ther 2017[Jun]; 19 (1 ): 136 PMID28615072 show ga BACKGROUND: Persistent expansion of circulating CD4(+) effector memory T cells (T(EM)) in patients with granulomatosis with polyangiitis (GPA) suggests their fundamental role in disease pathogenesis. Recent studies have shown that distinct functional CD4(+) T(EM) cell subsets can be identified based on expression patterns of chemokine receptors. The current study aimed to determine different CD4(+) T(EM) cell subsets based on chemokine receptor expression in peripheral blood of GPA patients. Identification of particular circulating CD4(+) T(EM) cells subsets may reveal distinct contributions of specific CD4(+) T(EM) subsets to the disease pathogenesis in GPA. METHOD: Peripheral blood of 63 GPA patients in remission and 42 age- and sex-matched healthy controls was stained immediately after blood withdrawal with fluorochrome-conjugated antibodies for cell surface markers (CD3, CD4, CD45RO) and chemokine receptors (CCR4, CCR6, CCR7, CRTh2, CXCR3) followed by flow cytometry analysis. CD4(+) T(EM) memory cells (CD3(+)CD4(+)CD45RO(+)CCR7(-)) were gated, and the expression patterns of chemokine receptors CXCR3(+)CCR4(-)CCR6(-)CRTh2(-), CXCR3(-)CCR4(+)CCR6(-)CRTh2(+), CXCR3(-)CCR4(+)CCR6(+)CRTh2(-), and CXCR3(+)CCR4(-)CCR6(+)CRTh2(-) were used to distinguish T(EM)1, T(EM)2, T(EM)17, and T(EM)17.1 cells, respectively. RESULTS: The percentage of CD4(+) T(EM) cells was significantly increased in GPA patients in remission compared to HCs. Chemokine receptor co-expression analysis within the CD4(+) T(EM) cell population demonstrated a significant increase in the proportion of T(EM)17 cells with a concomitant significant decrease in the T(EM)1 cells in GPA patients compared to HC. The percentage of T(EM)17 cells correlated negatively with T(EM)1 cells in GPA patients. Moreover, the circulating proportion of T(EM)17 cells showed a positive correlation with the number of organs involved and an association with the tendency to relapse in GPA patients. Interestingly, the aberrant distribution of T(EM)1 and T(EM)17 cells is modulated in CMV- seropositive GPA patients. CONCLUSIONS: Our data demonstrates the identification of different CD4(+) T(EM) cell subsets in peripheral blood of GPA patients based on chemokine receptor co-expression analysis. The aberrant balance between T(EM)1 and T(EM)17 cells in remission GPA patients, showed to be associated with disease pathogenesis in relation to organ involvement, and tendency to relapse. |Adult [MESH] |Aged [MESH] |Aged, 80 and over [MESH] |Female [MESH] |Granulomatosis with Polyangiitis/*immunology [MESH] |Humans [MESH] |Immunologic Memory/*immunology [MESH] |Male [MESH] |Middle Aged [MESH] |T-Lymphocyte Subsets/*immunology [MESH]Chemokine receptor co-expression reveals aberrantly distributed T(H) e(epmc).pdf DeepDyve Pubget Overpricing