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10.1615/CritRevEukaryotGeneExpr.2016015724

http://scihub22266oqcxt.onion/10.1615/CritRevEukaryotGeneExpr.2016015724
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C5470591!5470591!27480773
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suck abstract from ncbi

pmid27480773      Crit+Rev+Eukaryot+Gene+Expr 2016 ; 26 (2): 97-113
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  • Clinical Genomics: Challenges and Opportunities #MMPMID27480773
  • Vijay P; McIntyre AB; Mason CE; Greenfield JP; Li S
  • Crit Rev Eukaryot Gene Expr 2016[]; 26 (2): 97-113 PMID27480773show ga
  • Next-generation sequencing (NGS) approaches are highly applicable to clinical studies. We review recent advances in sequencing technologies, as well as their benefits and tradeoffs, to provide an overview of clinical genomics from study design to computational analysis. Sequencing technologies enable genomic, transcriptomic, and epigenomic evaluations. Studies that use a combination of whole genome, exome, mRNA, and bisulfite sequencing are now feasible due to decreasing sequencing costs. Single-molecule sequencing increases read length, with the MinION? nanopore sequencer, which offers a uniquely portable option at a lower cost. Many of the published comparisons we review here address the challenges associated with different sequencing methods. Overall, NGS techniques, coupled with continually improving analysis algorithms, are useful for clinical studies in many realms, including cancer, chronic illness, and neurobiology. We, and others in the field, anticipate the clinical use of NGS approaches will continue to grow, especially as we shift into an era of precision medicine.
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