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10.1186/s13059-017-1237-8 http://scihub22266oqcxt.onion/10.1186/s13059-017-1237-8 C5470253!5470253!28615073     free     free     free Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Genome+Biol 2017 ; 18 (ä): ä Nephropedia Template TP {{tp|p=28615073|t=2017. CRISPR/Cas9-mediated genome editing induces exon skipping by alternative splicing or exon deletion |pdf=|usr=}}{{28615073}} gab.com Text CRISPR/Cas9-mediated genome editing induces exon skipping by alternative splicing or exon deletion JO: Genome Biol http://www.kidney.de/pget.php?&tw=1&pmid=28615073 #FOAvip CRISPR is widely used to disrupt gene function by inducing small insertions and deletions. Here, we show that some single-guide RNAs (sgRNAs) can induce exon skipping or large genomic deletions that delete exons. For example, CRISPR-mediated editing of ?-catenin exon 3, which encodes an autoinhibitory domain, induces partial skipping of the in-frame exon and nuclear accumulation of ?-catenin. A single sgRNA can induce small insertions or deletions that partially alter splicing or unexpected larger deletions that remove exons. Exon skipping adds to the unexpected outcomes that must be accounted for, and perhaps taken advantage of, in CRISPR experiments.Electronic supplementary material: The online version of this article (doi:10.1186/s13059-017-1237-8) contains supplementary material, which is available to authorized users. Twit Text FOAVip CRISPR/Cas9-mediated genome editing induces exon skipping by alternative splicing or exon deletion ????Genome Biol http://www.kidney.de/pget.php?&tw=1&pmid=28615073 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28615073 #FOAvip English Wikipedia <ref>{{Cite pmid|28615073}}</ref> CRISPR/Cas9-mediated genome editing induces exon skipping by alternative splicing or exon deletion #MMPMID28615073 Mou H; Smith JL; Peng L; Yin H; Moore J; Zhang XO; Song CQ; Sheel A; Wu Q; Ozata DM; Li Y; Anderson DG; Emerson CP; Sontheimer EJ; Moore MJ; Weng Z; Xue W Genome Biol 2017[]; 18 (ä): ä PMID28615073show ga CRISPR is widely used to disrupt gene function by inducing small insertions and deletions. Here, we show that some single-guide RNAs (sgRNAs) can induce exon skipping or large genomic deletions that delete exons. For example, CRISPR-mediated editing of ?-catenin exon 3, which encodes an autoinhibitory domain, induces partial skipping of the in-frame exon and nuclear accumulation of ?-catenin. A single sgRNA can induce small insertions or deletions that partially alter splicing or unexpected larger deletions that remove exons. Exon skipping adds to the unexpected outcomes that must be accounted for, and perhaps taken advantage of, in CRISPR experiments.Electronic supplementary material: The online version of this article (doi:10.1186/s13059-017-1237-8) contains supplementary material, which is available to authorized users. äCRISPR/Cas9-mediated genome editing induces exon skipping by alternati(epmc).pdf DeepDyve Pubget Overpricing