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10.1038/ncomms15370 http://scihub22266oqcxt.onion/10.1038/ncomms15370 C5467245!5467245!28589952     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Commun 2017 ; 8 (ä): ä Nephropedia Template TP {{tp|p=28589952|t=2017. Contrasting mechanisms of growth in two model rod-shaped bacteria |pdf=|usr=}}{{28589952}} gab.com Text Contrasting mechanisms of growth in two model rod-shaped bacteria JO: Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=28589952 #FOAvip How cells control their shape and size is a long-standing question in cell biology. Many rod-shaped bacteria elongate their sidewalls by the action of cell wall synthesizing machineries that are associated to actin-like MreB cortical patches. However, little is known about how elongation is regulated to enable varied growth rates and sizes. Here we use total internal reflection fluorescence microscopy and single-particle tracking to visualize MreB isoforms, as a proxy for cell wall synthesis, in Bacillus subtilis and Escherichia coli cells growing in different media and during nutrient upshift. We find that these two model organisms appear to use orthogonal strategies to adapt to growth regime variations: B. subtilis regulates MreB patch speed, while E. coli may mainly regulate the production capacity of MreB-associated cell wall machineries. We present numerical models that link MreB-mediated sidewall synthesis and cell elongation, and argue that the distinct regulatory mechanism employed might reflect the different cell wall integrity constraints in Gram-positive and Gram-negative bacteria. Twit Text FOAVip Contrasting mechanisms of growth in two model rod-shaped bacteria ????Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=28589952 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28589952 #FOAvip English Wikipedia <ref>{{Cite pmid|28589952}}</ref> Contrasting mechanisms of growth in two model rod-shaped bacteria #MMPMID28589952 Billaudeau C; Chastanet A; Yao Z; Cornilleau C; Mirouze N; Fromion V; Carballido-López R Nat Commun 2017[]; 8 (ä): ä PMID28589952show ga How cells control their shape and size is a long-standing question in cell biology. Many rod-shaped bacteria elongate their sidewalls by the action of cell wall synthesizing machineries that are associated to actin-like MreB cortical patches. However, little is known about how elongation is regulated to enable varied growth rates and sizes. Here we use total internal reflection fluorescence microscopy and single-particle tracking to visualize MreB isoforms, as a proxy for cell wall synthesis, in Bacillus subtilis and Escherichia coli cells growing in different media and during nutrient upshift. We find that these two model organisms appear to use orthogonal strategies to adapt to growth regime variations: B. subtilis regulates MreB patch speed, while E. coli may mainly regulate the production capacity of MreB-associated cell wall machineries. We present numerical models that link MreB-mediated sidewall synthesis and cell elongation, and argue that the distinct regulatory mechanism employed might reflect the different cell wall integrity constraints in Gram-positive and Gram-negative bacteria. äContrasting mechanisms of growth in two model rod-shaped bacteria (epmc).pdf DeepDyve Pubget Overpricing