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10.1016/j.redox.2017.05.019 http://scihub22266oqcxt.onion/10.1016/j.redox.2017.05.019 C5466588!5466588 !28600983     free     free     free Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\28600983 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Redox+Biol 2017 ; 13 (ä): 266-277 Nephropedia Template TP {{tp|p=28600983 |t=2017. HPW-RX40 prevents human platelet activation by attenuating cell surface protein disulfide isomerases |pdf=|usr=}}{{28600983 }} gab.com Text HPW-RX40 prevents human platelet activation by attenuating cell surface protein disulfide isomerases JO: Redox Biol http://www.kidney.de/pget.php?&tw=1&pmid=28600983 #FOAvip Protein disulfide isomerase (PDI) present at platelet surfaces has been considered to play an important role in the conformational change and activation of the integrin glycoprotein IIb/IIIa (GPIIb/IIIa) and thus enhances platelet aggregation. Growing evidences indicated that platelet surface PDI may serve as a potential target for developing of a new class of antithrombotic agents. In the present study, we investigated the effects of HPW-RX40, a chemical derivative of ?-nitrostyrene, on platelet activation and PDI activity. HPW-RX40 inhibited platelet aggregation, GPIIb/IIIa activation, and P-selectin expression in human platelets. Moreover, HPW-RX40 reduced thrombus formation in human whole blood under flow conditions, and protects mice from FeCl(3)-induced carotid artery occlusion. HPW-RX40 inhibited the activity of recombinant PDI family proteins (PDI, ERp57, and ERp5) as well as suppressed cell surface PDI activity of platelets in a reversible manner. Exogenous addition of PDI attenuated the inhibitory effect of HPW-RX40 on GPIIb/IIIa activation. Structure-based molecular docking simulations indicated that HPW-RX40 binds to the active site of PDI by forming hydrogen bonds. In addition, HPW-RX40 neither affected the cell viability nor induced endoplasmic reticulum stress in human cancer A549 and MDA-MB-231 cells. Taken together, our results suggest that HPW-RX40 is a reversible and non-cytotoxic PDI inhibitor with antiplatelet effects, and it may have a potential for development of novel antithrombotic agents. Twit Text FOAVip HPW-RX40 prevents human platelet activation by attenuating cell surface protein disulfide isomerases ????Redox Biol http://www.kidney.de/pget.php?&tw=1&pmid=28600983 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28600983 #FOAvip English Wikipedia <ref>{{Cite pmid|28600983 }}</ref> HPW-RX40 prevents human platelet activation by attenuating cell surface protein disulfide isomerases #MMPMID28600983 Kung PH ; Hsieh PW ; Lin YT ; Lee JH ; Chen IH ; Wu CC Redox Biol 2017[Oct]; 13 (ä): 266-277 PMID28600983 show ga Protein disulfide isomerase (PDI) present at platelet surfaces has been considered to play an important role in the conformational change and activation of the integrin glycoprotein IIb/IIIa (GPIIb/IIIa) and thus enhances platelet aggregation. Growing evidences indicated that platelet surface PDI may serve as a potential target for developing of a new class of antithrombotic agents. In the present study, we investigated the effects of HPW-RX40, a chemical derivative of ?-nitrostyrene, on platelet activation and PDI activity. HPW-RX40 inhibited platelet aggregation, GPIIb/IIIa activation, and P-selectin expression in human platelets. Moreover, HPW-RX40 reduced thrombus formation in human whole blood under flow conditions, and protects mice from FeCl(3)-induced carotid artery occlusion. HPW-RX40 inhibited the activity of recombinant PDI family proteins (PDI, ERp57, and ERp5) as well as suppressed cell surface PDI activity of platelets in a reversible manner. Exogenous addition of PDI attenuated the inhibitory effect of HPW-RX40 on GPIIb/IIIa activation. Structure-based molecular docking simulations indicated that HPW-RX40 binds to the active site of PDI by forming hydrogen bonds. In addition, HPW-RX40 neither affected the cell viability nor induced endoplasmic reticulum stress in human cancer A549 and MDA-MB-231 cells. Taken together, our results suggest that HPW-RX40 is a reversible and non-cytotoxic PDI inhibitor with antiplatelet effects, and it may have a potential for development of novel antithrombotic agents. |*Platelet Activation [MESH] |Animals [MESH] |Binding Sites [MESH] |Blood Platelets/*drug effects/immunology [MESH] |Cell Line, Tumor [MESH] |Chlorobenzoates/*pharmacology [MESH] |Enzyme Inhibitors/*pharmacology [MESH] |Humans [MESH] |Male [MESH] |Mice [MESH] |Mice, Inbred C57BL [MESH] |Molecular Docking Simulation [MESH] |Protein Binding [MESH] |Protein Disulfide-Isomerases/antagonists & inhibitors/chemistry/*metabolism [MESH]HPW-RX40 prevents human platelet activation by attenuating cell surfac(epmc).pdf DeepDyve Pubget Overpricing