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10.3389/fcimb.2017.00249 http://scihub22266oqcxt.onion/10.3389/fcimb.2017.00249 C5465278!5465278!28649561     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Front+Cell+Infect+Microbiol 2017 ; 7 (ä): ä Nephropedia Template TP {{tp|p=28649561|t=2017. Psd1 Effects on Candida albicans Planktonic Cells and Biofilms |pdf=|usr=}}{{28649561}} gab.com Text Psd1 Effects on Candida albicans Planktonic Cells and Biofilms JO: Front Cell Infect Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=28649561 #FOAvip Candida albicans is an important human pathogen, causing opportunistic infections. The adhesion of planktonic cells to a substrate is the first step for biofilm development. The antimicrobial peptide (AMP) Psd1 is a defensin isolated from Pisum sativum seeds. We tested the effects of this AMP on C. albicans biofilms and planktonic cells, comparing its activity with amphotericin B and fluconazole. Three C. albicans variants were studied, one of them a mutant deficient in glucosylceramide synthase, conferring resistance to Psd1 antifungal action. Atomic force microscopy (AFM) was used to assess morphological and biomechanical changes on fungal cells. Surface alterations, with membrane disruption and leakage of cellular contents, were observed. Cytometry assays and confocal microscopy imaging showed that Psd1 causes cell death, in a time and concentration-dependent manner. These results demonstrate Psd1 pleiotropic action against a relevant fungal human pathogen, suggesting its use as natural antimycotic agent. Twit Text FOAVip Psd1 Effects on Candida albicans Planktonic Cells and Biofilms ????Front Cell Infect Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=28649561 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28649561 #FOAvip English Wikipedia <ref>{{Cite pmid|28649561}}</ref> Psd1 Effects on Candida albicans Planktonic Cells and Biofilms #MMPMID28649561 Gonçalves S; Silva PM; Felício MR; de Medeiros LN; Kurtenbach E; Santos NC Front Cell Infect Microbiol 2017[]; 7 (ä): ä PMID28649561show ga Candida albicans is an important human pathogen, causing opportunistic infections. The adhesion of planktonic cells to a substrate is the first step for biofilm development. The antimicrobial peptide (AMP) Psd1 is a defensin isolated from Pisum sativum seeds. We tested the effects of this AMP on C. albicans biofilms and planktonic cells, comparing its activity with amphotericin B and fluconazole. Three C. albicans variants were studied, one of them a mutant deficient in glucosylceramide synthase, conferring resistance to Psd1 antifungal action. Atomic force microscopy (AFM) was used to assess morphological and biomechanical changes on fungal cells. Surface alterations, with membrane disruption and leakage of cellular contents, were observed. Cytometry assays and confocal microscopy imaging showed that Psd1 causes cell death, in a time and concentration-dependent manner. These results demonstrate Psd1 pleiotropic action against a relevant fungal human pathogen, suggesting its use as natural antimycotic agent. äPsd1 Effects on Candida albicans Planktonic Cells and Biofilms (epmc).pdf DeepDyve Pubget Overpricing